Thiophene-2-ethylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: A non GLP study according to acceptable guideline.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Norme française AFNOR T90 301 de janvier 1983 intitulée: "ESSAIS DES EAUX"

Principles of method if other than guideline:

Determination of inhibition of the mobility of Daphnia magna following the standard method described in AFNOR T 90 301.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Thiophene-2-ethylamine

EC Number:

250-196-9

EC Name:

Thiophene-2-ethylamine

Cas Number:

30433-91-1

Molecular formula:

C6H9NS

IUPAC Name:

2-(2-thienyl)ethanamine

Constituent 2

Reference substance name:

2-Thiopheneethanamine

IUPAC Name:

2-Thiopheneethanamine

Details on test material:

- Name of test material (as cited in study report): Intermediare clopidogral (S3285)
- Lot/batch No.: 4SNS002

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

Preparation of standard solution: 250 mg of product in 250 mL of test medium

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna Straus, 1820 (Clasdocera crustacea) aged less than 24h.
Origine: INERIS laboratory

Study design

Test type:

static

Water media type:

not specified

Total exposure duration:

48 h

Remarks on exposure duration:

in obscurity

Post exposure observation period:

Not applicable

Test conditions

Hardness:

No data

Test temperature:

20°C +/- 2°C

pH:

pH of the solution at 1 g/L = 10.02

Dissolved oxygen:

> 2 mg/L

Salinity:

No data

Nominal and measured concentrations:

6.8 - 9 - 13 - 19 - 26 - 36 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass tube
- fill volume: each glass tube contains 10 mL (2mL of solution for each daphnia)
- Aeration: none
- No. of organisms per vessel: 5 daphnids
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Feeding: none

TEST MEDIUM / WATER PARAMETERS
- Preparation of medium: The test medium is prepared from known analytical grade reagents
- pH: 8.0 +/- 0.2
- Conductivity: 700 +/- 20 µS/cm

OTHER TEST CONDITIONS
- Adjustment of pH:
- Photoperiod:
- Light intensity: obscurity

EFFECT PARAMETERS MEASURED: effective initial concentration that that immobilizes in 48h, 50% of the population of Daphnia magna.

TEST CONCENTRATIONS
- Test concentrations: 6.8 - 9 - 13 - 19 - 269 - 36 mg/L
- Results used to determine the conditions for the definitive study:

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

12.99 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95% confidence limit

Details on results:

EC50-48h determined by the method of calculation of PROBITS (confidence limits 95%): 12.99 mg/l (11.8-14.96 mg/l).

Results with reference substance (positive control):

EC50-24h determined for potassium dichromate by the method of calculation of PROBITS (confidence limits 95%):
1.32 mg/l (1.19-1.48 mg/l): week of 24-28 october 1994

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

In an acute toxicity test with daphnia the EC50 of the test substance was found 12.99 mg/l at 48 h.