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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 July 2017-14 August 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
yes
Details on sampling:
A stock solution of the test item was prepared by dissolving 0.020 g of the test item (nominal; actually weighed amounts were 0.0200-0.0203 g) in 2000 mL of test medium, resulting in a nominal concentration of 10 mg test item/L. This stock solution was stirred for 1-2 h at ambient temperature in the dark. The stock solution was clear, without Tyndall effect, and used, undiluted, as the limit test concentration (10 mg/L).
Preparation of the stock solution (test solution) was repeated on days 5, 8, and 14 of exposure.
Vehicle:
no
Details on test solutions:
The test vessels containing the test solutions were placed into a temperature controlled room at test temperature for temperature adaptation. The test vessels placed on three trays that were arranged in the testing area in a randomised manner at start of the test and after each renewal of test solutions.
Test organisms (species):
Daphnia magna
Test type:
semi-static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
21 d
Remarks on exposure duration:
The measured concentrations were stable over the exposure period (≥90% of nominal). All biological results are therefore based on nominal concentrations.
Post exposure observation period:
Not relevant
Hardness:
14.2 (°dH)
Test temperature:
22.5-22.7°C
pH:
7.6-8.1
Dissolved oxygen:
8.4-8.3 mg/L
Salinity:
No information
Conductivity:
676-677 µS/cm
Nominal and measured concentrations:
All measured concentrations were ≥ 90% of nominal. This indicates that the test item was stable in the test solutions. All biological results are therefore based on nominal concentrations.
Details on test conditions:
Summary of the physical-chemical parameters measured during the test period.
Parameter Unit Minimum Maximum
pH [–] 7.7 8.8
Dissolved oxygen [% of air sat. value] 99 116
Dissolved oxygen [mg/L] 8.7 10.0
Temperature [°C] 19.7 21.0
"Temperature
(automatic measurement)" [°C] 19.4 21.2
water hardness [°dH] 14.0 14.2
water hardness [mg CaCO3/L] [a] 250 254
(manual measurements)
[a] Water hardness in mg CaCO3/L was calculated by multiplication of the hardness-value in °dH by 17.86.
Reference substance (positive control):
no
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Key result
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Key result
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (total fraction)
Basis for effect:
growth

Nominal concentration Mean living offspring [n] Adult survival Mean age at first reproduction [d] Mean adult length [mm] Mean intrinsic rate r
[mg/L] mean (sd) [% of initial number] mean (sd) mean (sd) mean (sd)
Control 90.0 (23.9) 100 10.5 (1.33) 4.58 (0.320) 0.305 (0.0302)
10.0 89.3 (26.9) 100 11.4 (0.88) 4.52 (0.327) 0.294 (0.0448)
Validity criteria fulfilled:
yes
Conclusions:
At the concentration of 10 mg/L, no toxicity was observed during the test neither on reproduction/fecundity aspects, nor in Mortality/immobility of parental daphnids, and on length of parental dapnids.
Executive summary:

The NOEC has been derermined at 10 mg/L, and a LOEC can been assessed ar a value higher than 10 mg/L.

Description of key information

Based on a valid OECD 211 semi-static test on Daphnia magna reproduction, no specific concentration-response relation ship of daphnid reproduction was observed for the test item. Therefore based on a limit test a NOEC has been deterrmined ( for reproductio/fecundity, Mortality and Lenght of parental daphnids) at 10 mg//L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
10 mg/L

Additional information