2,2-dimethyl-1,3-dioxolan-4-ylmethanol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

September, 2013 to May, 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD Guideline and with GLP compliance

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Cambridge Wastewater Treatment Facility, Cambridge (Maryland), collected on 07 October, 2013.
- Laboratory culture: not applicable
- Method of cultivation: wastewater treatment plant
- Storage conditions: no data
- Pretreatment: sieved through a 2 mm screen, aerated overnight, and washed twice with tap water.
- Concentration of sludge: 2500 mg/L
- Initial cell/biomass concentration: no data
- Water filtered: yes
- Type and size of filter used, if any: 2mm screen

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

COD

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

Aliquots of the test solution were removed from each of the test chambers at 3 hours, and days 1, 3, 7, 14, 21, 27 and 28. The samples were centrifuged at approximately 2500 rpm for 10 minutes and the resultant supernatants were filtered through 0.45 m filters. Dissolved total carbon and dissolved inorganic carbon measurements were performed using a Shimadzu TOC analyzer, Model TOC Vcsh to provide dissolved organic carbon (DOC) values. On each sampling day, the pH of the test solutions were measured and adjusted to pH 6.5-8.0 if necessary.

Reference substance:

diethylene glycol

Remarks:

Name: Diethylene Glycol BioUltra ≥ 99.0% (GC) Manufacturer: Sigma-Aldrich Lot Number: bcbk7135V Physical Description: Liquid Chemical Abstract Number: 111-46-6 Purity: ≥99.0% Storage Conditions: Ambient

Parameter:

% degradation (DOC removal)

Value:

25

Sampling time:

28 d

Results with reference substance:

The reference substance, diethylene glycol, degrading an average of 100.0% (nominal concentration of 250mg/L). The reference substance degraded greater than 70% by day 14 of the test, thereby fulfilling the criteria for a valid test.
The toxicity control degraded > 25% by Day 14 of the study, allowing for the test substance to be considered non-inhibitory at the concentration tested.

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

Augeo SL 191 may be considered inherent, primary biodegradability since greater than 20% loss of DOC was observed.

Executive summary:

The inherent biodegradability of 2,2-DIMETHYL-1,3-DIOXOLAN-4-YLMETHANOL was determined by the Zahn Wellens/EMPA Test (OECD Guideline 302B). Degradability was based on the dissolved organic carbon (DOC) concentration of the test solutions. The reference chambers were dosed with diethylene glycol, a substance known to be biodegradable, at a nominal concentration of 250 mg C/L. The treatment group test chambers were used to evaluate Augeo SL 191 at a nominal concentration of 250 mg C/L. The toxicity control was used to evaluate toxicity of the test substance to the inoculum and was dosed with both the reference (250 mg C/L) and test substances (250 mg C/L).

The results indicated that the activated sludge inoculum was active, degrading the reference substance an average of 100.0%. The average cumulative percent biodegradation for Augeo SL 191 was 25.0%. The toxicity control degraded 56.1%, indicating that the test substance was not inhibitory in the test system at the concentration tested.

may be considered

2,2-DIMETHYL-1,3-DIOXOLAN-4-YLMETHANOL may be considered as inherently, primary biodegradable.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April, 2014 to June, 2014

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD 301D guideline and with GLP compliance

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic, non-adapted

Details on inoculum:

The source of test organisms was secondary effluent freshly obtained from a municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.

Duration of test (contact time):

28 d

Initial conc.:

1 mg/L

Based on:

test mat.

Initial conc.:

3 mg/L

Based on:

test mat.

Details on study design:

Test set up:
- Inoculum blank Containing only inoculum (no test substance)
- Procedure control Containing reference substance and inoculum
- Test suspension Containing test substance and inoculum (applicable for both test concentrations)
- Toxicity control Containing test substance at the lowest concentration, reference substance and inoculum.

Number and groups of test bottles : Individual BOD bottles were prepared for each measuring point, except that the bottles measured at the start were also measured on day 7. Parallel groups were prepared to allow duplicate measurements of oxygen consumption at the test intervals.

Reference substance:

acetic acid, sodium salt

Remarks:

- Batch number K34333668 - Article number 1.06268.0250 - Purity >99.0% - Expiry Date : 28 February 2015 - Storage conditions At room temperature in the dark - Supplier Merck, Darmstadt, Germany

Parameter:

% degradation (O2 consumption)

Value:

4

Sampling time:

28 d

Results with reference substance:

In the toxicity control more than 25% biodegradation occurred within 14 days (34%, based on ThOD). Thus, the toxicity control showed that AUGEO SL 191 did not inhibit microbial activity.
The control substance was degraded by at least 60% (at least 75%, mean value) within 14 days.

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

AUGEO SL 191 was not readily biodegradable under the conditions of the OECD 301D closed bottle test performed.

Executive summary:

During 28 days, the biodegradation of Augeo SL191 at an initial concentration of 1 and 3 mg/L was studied according to OECD guideline 301D.

Inoculum used in the test was an domestic sewage non acclimated sludge at a concentration of 30 mg/L obtained with domestic sample from municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch.

The corresponding percentage of biodegradation obtained after 28 days was 4% (mean of the two concentrations tested).

Reference material (sodium acetate) validates the test since its biodegradation was greater than 60 % at 14 days (75%).

Under the test conditions Augeo SL191 is not readily biodegradable.

Description of key information

AUGEO SL 191 was not readily biodegradable under the conditions of the closed bottle test performed (OECD Guideline 301D).
Augeo SL191 may be considered as inherently, primary biodegradable (OECD Guideline 302B).

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater