cis-hex-3-en-1-ol

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 January 1996- 25 March 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GPL guideline study.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): City of Geneva, Aïre
- Storage conditions: aerobic
- Storage length: used on the same day as collected
- Preparation of inoculum for exposure: the sludge is collected in the morning and washed 3 times in the mineral medium by centrifuging at 1000 g for 10 min, discarding the supernatant and resuspending in mineral medium
- Concentration of sludge: 1.88 mL of sludge is needed to obtain a concentration of 30 mg/L (dry weight) in a 250 mL flask

Duration of test (contact time):

28 d

Initial conc.:

100 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Prepared by mixing 50 mL of stock solution A and 2000 mL deionised water, adding 5 mL of each of the stock solutions B, C and D and making up to 5 L with ionised water.
- Test temperature: 22 °C
- pH: 7.4 ± 0.2
- pH adjusted: Yes, with phosphoric acid or potassium hydroxide
- Suspended solids concentration: 3.985 g/L

TEST SYSTEM
- Number of culture flasks/concentration: duplicate flask per concentration
- Measuring equipment: The respirometer used during this study is a SAPROMAT D 12, made by J.M. VOITH GmbH, D-7920 Heidenheim.
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: soda lime

SAMPLING
Everyday the oxygen consumption of each flask is recorded and correct temperature and stirring are checked. At the end of the test period, the pH of each flask is measured again.

CONTROL AND BLANK SYSTEM
A pair of flasks of the volumetric respirometer (SAPROMAT) are filled with mineral medium + test chemical (100 mg/L) + aniline (100 mg/L) + inoculums and their respirations are recorded for the other flasks. If they are lower than those of the flasks containing mineral medium + aniline (100 mg/L) + inoculums the test chemical can be assumed to be inhibitory to the inoculums used.

Reference substance:

aniline

Remarks:

minimum purity 99.5 %

Test performance:

Degradation of the reference substance exceeded 40 % over 7 days and 65 % after 14 days: the activity of the inoculums was this verified and the test was considered valid.

Parameter:

% degradation (O2 consumption)

Value:

77

Sampling time:

28 d

Details on results:

The test substance underwent 77 % biodegradability after 28 days in the test conditions. Biodegradation started on day 1 and reached 72 % at the end of the 10 day window (days 1 to 11).

Results with reference substance:

Degradation of the reference substance exceeded 40 % over 7 days and 65 % after 14 days.

Oxygen uptakes, as read on the SAPROMAT meters, were corrected by deducting the basic oxygen uptake of sludge and proportionally to account for the differences between actual and nominal concentrations of test and reference substances.

Averages of identical flasks were used to obtain the graph of BOD versus time (attachment 1), percentage of biodegradation versus time (attachment 2) and biodegradation rates.

Biodegradability of the test substance- Respirometric Method

Theoretical oxygen demand:

Formula: C6H12N0O1

Molecular weight: 100.16 g/mol

Theoretical Oxygen Demand (ThOD): 2.72 mg O2/mg

Biodegradability:

Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations):

		Days:	1	7	11	14	21	28
BOD Sludge	1rst flask	B1	4.0	18.0	22.0	24.0	27.0	33.0
	2^ndflask	B2	1.0	17.0	20.0	22.0	25.0	31.0
	mean	B	2.5	17.5	21.0	23.0	26.0	32.0
BOD Sludge Test Subst.	1rst flask	C1	41.4	181.6	223.0	238.2	247.2	247.2
	2^ndflask	C2	38.6	177.5	209.9	228.2	238.3	238.2
	1rst fl. corr.	C1- B	38.9	164.1	202.0	215.2	221.2	215.2
	2nd fl. corr.	C2- B	36.1	160.0	188.9	205.2	212.3	206.2
% degr.	1rst flask	D1	14	60	74	79	81	79
	2^ndflask	D2	13	59	69	75	78	79
	mean	D	14	60	72	77	80	77

B= (B1 + B2)/ 2

D1= 100 * (C1 – B)/ ThOD * [S] where [S] is the initial test substance concentration (mg/L)

D2= 100 * (C2 – B)/ ThOD * [S]

D= (D1 + D2)/ 2

 

Biodegradability of the reference substance- Respirometric Method

Theoretical oxygen demand:

Formula: C6H7N1O0

Molecular weight: 93.13 g/mol

Theoretical Oxygen Demand (ThOD): 2.41 mg O2/mg

Biodegradability:

Biological Oxygen Demand (BOD, mg O2/L, adjusted to nominal concentrations):

		Days:	5	7	10	14	21	28
BOD Sludge	1rst flask	B1	16.0	18.0	21.0	24.0	27.0	33.0
	2^ndflask	B2	14.0	17.0	19.0	22.0	25.0	31.0
	mean	B	15.0	17.5	20.0	23.0	26.0	32.0
BOD Sludge Test Subst.	1rst flask	C1	184.0	199.0	209.0	215.0	220.0	229.0
	2^ndflask	C2	181.0	198.0	210.0	217.0	224.0	236.0
	1rst fl. corr.	C1- B	169.0	181.5	189.0	192.0	194.0	197.0
	2nd fl. corr.	C2- B	166.0	180.5	190.0	194.0	198.0	204.0
% degr.	1rst flask	D1	70	75	78	80	80	82
	2^ndflask	D2	69	75	79	80	82	85
	mean	D	70	75	79	80	81	83

B= (B1 + B2)/ 2

D1= 100 * (C1 – B)/ ThOD * [S] where [S] is the initial test substance concentration (mg/L)

D2= 100 * (C2 – B)/ ThOD * [S]

D= (D1 + D2)/ 2

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The ready biodegradability of the test substance was assessed according to OECD guideline 301 F. The test substance underwent 77 % biodegradation after 28 days in the test conditions. Biodegradation started on day 1 and reached 72 % at the end of the 10-day window (days 1 to 11). Therefore, the test substance can be regarded as readily biodegradable according to this test.

Executive summary:

The ready biodegradability of the test item was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 301 F.

The test item underwent 77 % biodegradation after 28 days in the test conditions. Biodegradation started on day 1 and reached 72 % at the end of the 10-day window (days 1 to 11).

Thus, the test item should be regarded as readily biodegradable.

At the concentration used in the test (100 mg/L), it is not inhibitory to the micro-organisms.

Description of key information

The ready biodegradability of the test substance was assessed according to OECD guideline 301 F. The test substance underwent 77 % biodegradation after 28 days in the test conditions. Biodegradation started on day 1 and reached 72 % at the end of the 10-day window (days 1 to 11). Therefore, the test substance can be regarded as readily biodegradable according to this test.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

The ready biodegradability of the test item was determined by the Manometric Respirometry Test according to the OECD Guidelines for Testing of Chemicals, Method No. 301 F. The test item underwent 77 % biodegradation after 28 days in the test conditions. Biodegradation started on day 1 and reached 72 % at the end of the 10-day window (days 1 to 11). Thus, the test item should be regarded as readily biodegradable. At the concentration used in the test (100 mg/L), it is not inhibitory to the micro-organisms.