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Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: early study report, but basic information given

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1968
Report date:
1968

Materials and methods

Principles of method if other than guideline:
internal BASF guidelines
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Trilon AS
IUPAC Name:
Trilon AS
Constituent 2
Chemical structure
Reference substance name:
Nitrilotriacetic acid
EC Number:
205-355-7
EC Name:
Nitrilotriacetic acid
Cas Number:
139-13-9
Molecular formula:
C6H9NO6
IUPAC Name:
2-[bis(carboxymethyl)amino]acetic acid

Test animals

Species:
rat
Strain:
other: US rats
Sex:
male/female

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: 2, 16 and 30% aqueous suspension with traganth
Details on oral exposure:
Observation period: 14 days
Doses:
200, 1600, 3200, 6400 mg/kg
No. of animals per sex per dose:
20
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 6 400 mg/kg bw
Mortality:
see table below
Clinical signs:
other: 3200 - 6400 mg/kg: immediately accelerated breathing, high-stepping gait, disturbed behaviour. 3 days thereafter no symptoms, then deterioration of health with crusty eyes and noses, intermittent breathing and with intensively ruffled fur. The animals wer
Gross pathology:
Sacrificed animals: no abnormalities detected.

Any other information on results incl. tables

Dose (mg/kg)   Concentration (%)  No. of animals  Dead animals after            
       1 hour  24 hours  48 hours  7 days  14 days
 6400  30  20  0/20  0/20  0/20  3/20  4/20
 3200  30  20  0/20  0/20  0/20  1/20  1/20
 1600  16  20  0/20  0/20  0/20  0/20  
 200  2  20  0/20  0/20  0/20  0/20  

Applicant's summary and conclusion

Interpretation of results:
practically nontoxic
Remarks:
Migrated information Criteria used for interpretation of results: EU

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