1H-Benz[e]indolium, 2-[2-[2-chloro-3-[2-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,1,3-trimethyl-, 4-methylbenzenesulfonate (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

420-680-6

EC Name:

-

Cas Number:

134127-48-3

Molecular formula:

C47 H47 Cl N2 O3 S

IUPAC Name:

2-{2-[2-chloro-3-(2-{1,3,3-trimethyl-1H,2H,3H-benzo[g]indol-2-ylidene}ethylidene)cyclohex-1-en-1-yl]ethenyl}-1,1,3-trimethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate

Sampling and analysis

Analytical monitoring:

yes

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

2.5mmol/l as CaCO3

Test temperature:

21°C

pH:

7.8 - 8.0

Dissolved oxygen:

8.4 - 8.5mg/l

Salinity:

Analytical grade salts were dissolved in purified water to obtain the following nominal concentrations:
- CaCl2.2H2O: 2.0mmol/l
- MgSO4.4H2O: 0.5mmol/l
- NaHCO3: 0.75mmol/l
- KCl: 0.075mmol/l

Nominal and measured concentrations:

Due to the limited water solubility of the test item, a supersaturated dispersion of the test item with a loading rate of 100mg/l was continuously stirred at room temperature in the dark over 48 hours. This stock dispersion was filtered and the undiluted filtrate with the maximum concentration of dissolved test item was used as the highest test medium concentration and as the stock solution for dilutions in a geometric series differing by a constant factor of 2. The analytically determined test item concentration in the analyzed test medium samples of the undiluted filtrate amounted to 0.06µg/l at the start of the test and 0.09µg/l at the end of the test. The mean measured test item concentration (calculated as the average over all measurements) was 0.07µg/l.

Reference substance (positive control):

yes

Remarks:

Potassium chromate is testsed as positive control at least once a year to demonstrate satisfactory test conditions.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 0.07 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: The 48-hour NOEC might even be higher, but concentrations in excess of the loading rate of 100mg/l have not been tested due to the limited water solubility of the test item in the used test water.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 0.07 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: The 48-hour EC0 might even be higher, but concentrations in excess of the loading rate of 100mg/l have not been tested due to the limited water solubility of the test item in the used test water.

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.07 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

mobility

Remarks on result:

other: EC50 > water solubility

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

no acute toxic efects on Daphnia magna up to the solubility limit