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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: 3a: Documentation insufficient for assessment

Data source

Reference
Reference Type:
secondary source
Title:
Salmonella mutagenicity tests: III. Results from the testing of 255 chemicals, Environ. Mol. Mutagen. 9(SUPPL.9):1-110
Author:
Zeiger,E, Anderson,B, Haworth,S, Lawlor,T, Mortelmans,K and Speck,W
Year:
1987
Bibliographic source:
CCRIS

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
No further details
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Thiophene
EC Number:
203-729-4
EC Name:
Thiophene
Cas Number:
110-02-1
Molecular formula:
C4H4S
IUPAC Name:
thiophene

Method

Target gene:
Histidine
Species / strainopen allclose all
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Species / strain / cell type:
S. typhimurium TA 97
Metabolic activation:
with and without
Metabolic activation system:
RAT and HAMSTER, LIVER, S-9, AROCLOR 1254
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO
Details on test system and experimental conditions:
METHOD OF APPLICATION: preincubation

Results and discussion

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The bacterial reverse mutation assay (Ames test) for thiophene was negative in all strains tested.