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Ecotoxicological information

Toxicity to microorganisms

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Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1994 - 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
activated sludge from wste water treatment plant Hildesheim; 2 x washed.
Dry weight:2.6 g/L
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20°C
pH:
7.29
Reference substance (positive control):
yes
Remarks:
Copper(II)sulfate pentahydrate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
EC50 = 114 mg/L
Validity criteria fulfilled:
yes
Conclusions:
The substance sowed no effect on activated sludge up to a test concentration of 1000 mg/L.
Executive summary:

The substance was tested for bacteria toxicity by a respiration inhibition test with not adapted activated sludge according to OECD guideline 209. Concentrations used were 100 - 180 - 320 - 580 - 1000 [mg/l]. As reference substance copper(II)sulfate pentahydrate was used. In none of the tested concentrations between 100 and 1000 mg/L arespiration inhibition could be observed,

Description of key information

The substance sowed no effect on activated sludge up to a test concentration of 1000 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 000 mg/L

Additional information