Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1971

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

no guideline available

GLP compliance:

no

Test type:

other: dynamic and static nebulization inhalation

Test material

Test material information

Constituent 1

Reference substance name:

Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts

EC Number:

293-346-9

EC Name:

Naphthalenesulfonic acids, branched and linear Bu derivs., sodium salts

Cas Number:

91078-64-7

Molecular formula:

Not applicable (UVCB substance)

IUPAC Name:

Reaction products of naphtalene, butanol, sulfonated and neautralized by caustic soda

Test material form:

solid: particulate/powder

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

not specified

Vehicle:

other: water and Lutrol (1:1)

Details on inhalation exposure:

Dynamic nebulization inhalation
Tests were performed using an apparatus for dynamic inhalation developed by ourselves. The compound was nebulized in a mixture of water and Lutrol
Static nebulization inhalation
The animals were held in 2m3 rooms and subjected for 4 hours over half-hour periods to the active ingredient nebulized as a solution in water and Lutrol (1:1). The observation period lasted 14 days and the following results were obtained.

Duration of exposure:

4 h

Concentrations:

Dynamic
Male rats: 0.143, 0.400, 0.933 mg/L
Female rats: 0.180, 0.510, 0.940 mg/L

Static
0.108, 0.173, 0.864 mg/L

No. of animals per sex per dose:

20 rats/dose group in dynamic inhalation and 10 rats/dose group for static inhalation were used. The results were obtained over an observation period of 2 weeks.

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LC50

Effect level:

> 0.933 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 2

Sex:

female

Dose descriptor:

LC50

Effect level:

> 0.94 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Any other information on results incl. tables

Dynamic nebulization inhalation

Air concentration mg Erkantol BXG/1		Daily test period in hours	Number of test days	Tox. Result after 14 days
Theoretical	Analytically determined
male rats
1.0	0.143	4	1	0/0/20
2.5	0.400	4	1	0/20/20
5.0	0.933	4	1	5/20/20
female rats
1.0	0.180	4	1	0/0/20
2.5	0.510	4	1	0/20/20
5.0	0.940	4	1	0/20/20

LC50 male rat 4 h exposure > 0.933 mg/1 air

LC50 female rat 4 h exposure > 0.940 mg/1 air

At higher concentrations the animals exhibited a transient deterioration in their general health. The male rats were more sensitive than the females, and displayed labored breathing at the highest concentration

Static nebulization inhalation

Air concentration mg Erkantol BXG/1		Animal species	Tox. Result after 14 days
Theoretical	Analytically determined
1.0	0.108	rats	0/0/10
2.0	0.173	rats	0/10/10
5.0	0.864	rats	1/10/10

There were no irritant effects to the mucosa of the eye and nasal region.

Applicant's summary and conclusion

Interpretation of results:

other: classified as harmful (Acute Tox.4) according to the CLP Regulation (EC) No.1272/2008

Conclusions:

For the dynamic inhalation the LC50 is > 0.933 mg/l for male rats and >0.94 mg/l for female rats.

Executive summary:

The toxicity of the substance after exposure through the inhalation route was evaluated. Tests were performed using an apparatus for dynamic inhalation; the compound was nebulized in a mixture of water and Lutrol. The animals were held in 2m3 rooms and subjected for 4 hours over half-hour periods to the active ingredient nebulized as a solution in water and Lutrol (1:1). The observation period lasted 14 days.

During the Dynamic nebulization inhalation male rats were exposed at 0.143, 0.400, 0.933 mg/l, while female rats were exposed at 0.180, 0.510, 0.940 mg/L

During the static nebulization inhalation, rats were exposed at 0.108, 0.173, 0.864 mg/l.

For the dynamic inhalation the LC50 is > 0.933 mg/l for male rats and >0.94 for female rats which correspond to the highest concentration to which the animals were exposed.