Triclocarban

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.6200 (Earthworm Subchronic Toxicity Test)

Principles of method if other than guideline:

Study was conducted to determine the effect of test chemical on Eisenia fetida

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

No Data

Vehicle:

yes

Details on preparation and application of test substrate:

No Data

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name:Eisenia fetida

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

NO data

Test temperature:

22 Deg C

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

40 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: Other details not known

Details on results:

No data

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

not specified

Conclusions:

The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

Executive summary:

Study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

Description of key information

The first study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

40 mg/kg soil dw

Additional information

Toxicity to soil microorganisms except arthropods:

Summarized result for the toxicity of test chemical were studied on the soil microorganisms. The studies are as mention below:

 

The first study was conducted to determine the effect of test chemical on Eisenia fetida. The Lethal effect concentration (LC50) value of test chemical to Eisenia fetida in a 48 hrs study was determine to be 40 mg/kg.

 

First study was supported by the second study from peer reviewed journal. The effects of test chemical were studied in the freshwater mudsnail Potamopyrgus antipodarum over 4 weeks were determined. Aquatic vegetation was collected in D-nets, and snails were removed and kept in river water in 1- gallon buckets for transfer to the laboratory. Within 2 h, snails were transferred into 10-gallon aquaria filled with artificial freshwater (Milli-Q water plus added salts at a rate of 5 g CaCO3, 5 g sea salt, and 0.5 g NaHCO3 per 10 gallons of water). After 3 to 5 d of acclimation to laboratory conditions, 60 individuals with shell lengths greater than 3.0 mm were transferred to each individual 1-L jar filled with 800 ml artificial freshwater. The jars were aerated through glass pipettes to keep dissolved oxygen near saturation. After four weeks, environmentally relevant test chemical concentrations of 1.6 to 10.5 mg/L resulted in statistically significant increases in the number of unshelled embryos, whereas 0.2, 1.6, and 10.5 mg/L exposures significantly increased numbers of shelled embryos. The lowest observed effect concentration (LOEC) was 0.2 mg/L, the no observed effect concentration (NOEC) was 0.05 mg/L; the 10% effective concentration (EC10) and the median effective concentration (EC50) for unshelled effects were 0.5 mg/L and 2.5 mg/L, respectively.