Triclocarban

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Acute and chronic (or sub-chronic) toxicity of test chemical on fish Oryzias latipes were studied under the static system.

GLP compliance:

no

Analytical monitoring:

yes

Details on sampling:

No data

Vehicle:

yes

Details on test solutions:

No data

Test organisms (species):

Oryzias latipes

Details on test organisms:

Fish acute toxicity was tested using Japanese medaka (Oryzias latipes) purchased from the National Institute for Environmental Studies and acclimated in the laboratory of the University of Tokushima at least for 3 months. The acute tests were conducted in conformity to the OECD guideline for testing chemicals no. 203. Duplicates of five juvenile fish were prepared in a 100 ml beaker for at least five concentrations and blanks (and solvent blanks for TCC). The test was conducted at 25 °C with a photoperiod of 16 h light and 8 h dark. The solution was replaced at 48 h and the concentrations of the tested antimicrobials were analyzed using the HPLC presented above. The DO and pH of the solution were checked at 0, 48, and 96 h. The number of dead individuals was counted at 96 h and the median lethal concentration (LC50) was determined on the basis of geometrical mean of the measured concentrations of the tested antimicrobials using probit or logit conversion with Ecotox-Statics version 2.6d.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

25 °C

pH:

NO data

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.085 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Other details not known

Details on results:

No data

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

No data

Validity criteria fulfilled:

not specified

Conclusions:

The Lethal effect concentration (LC50) value of test chemical in fish in a 96 hr study on the basis of mortality effect was observed to be 0.085 mg/L.

Executive summary:

Acute toxicity of test chemical on fish Oryzias latipes were studied under the static system. Static system were used for the study. Fish acute toxicity was tested using Japanese medaka (Oryzias latipes) purchased from the National Institute for Environmental Studies and acclimated in the laboratory of the University of Tokushima at least for 3 months. The acute tests were conducted in conformity to the OECD guideline for testing chemicals no. 203. Duplicates of five juvenile fish were prepared in a 100 ml beaker for at least five concentrations and blanks (and solvent blanks for TCC). The test was conducted at 25 °C with a photoperiod of 16 h light and 8 h dark. The solution was replaced at 48 h and the concentrations of the tested antimicrobials were analyzed using the HPLC. The DO and pH of the solution were checked at 0, 48, and 96 h. The number of dead individuals was counted at 96 h and the median lethal concentration (LC50) was determined on the basis of geometrical mean of the measured concentrations of the tested antimicrobials using probit or logit conversion with Ecotox-Statics version 2.6d. The Lethal effect concentration (LC50) value of test chemical in fish in a 96 hr study on the basis of mortality effect was observed to be 0.085 mg/L. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1 category as per the CLP classification criteria.

Description of key information

Acute toxicity of test chemical on fish Oryzias latipes were studied under the static system. Static system were used for the study. Fish acute toxicity was tested using Japanese medaka (Oryzias latipes) purchased from the National Institute for Environmental Studies and acclimated in the laboratory of the University of Tokushima at least for 3 months. The acute tests were conducted in conformity to the OECD guideline for testing chemicals no. 203. Duplicates of five juvenile fish were prepared in a 100 ml beaker for at least five concentrations and blanks (and solvent blanks for TCC). The test was conducted at 25 °C with a photoperiod of 16 h light and 8 h dark. The solution was replaced at 48 h and the concentrations of the tested antimicrobials were analyzed using the HPLC. The DO and pH of the solution were checked at 0, 48, and 96 h. The number of dead individuals was counted at 96 h and the median lethal concentration (LC50) was determined on the basis of geometrical mean of the measured concentrations of the tested antimicrobials using probit or logit conversion with Ecotox-Statics version 2.6d. The Lethal effect concentration (LC50) value of test chemical in fish in a 96 hr study on the basis of mortality effect was observed to be 0.085 mg/L. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1 category as per the CLP classification criteria.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

0.085 mg/L

Additional information

Based on the short term toxicity study available for the test chemical, studies has were reviewed to determine the toxic nature of test chemical on the mortality of fish. The studies are as mentioned below:

Acute toxicity of test chemical on fish Oryzias latipes were studied under the static system. Static system were used for the study. Fish acute toxicity was tested using Japanese medaka (Oryzias latipes) purchased from the National Institute for Environmental Studies and acclimated in the laboratory of the University of Tokushima at least for 3 months. The acute tests were conducted in conformity to the OECD guideline for testing chemicals no. 203. Duplicates of five juvenile fish were prepared in a 100 ml beaker for at least five concentrations and blanks (and solvent blanks for TCC). The test was conducted at 25 °C with a photoperiod of 16 h light and 8 h dark. The solution was replaced at 48 h and the concentrations of the tested antimicrobials were analyzed using the HPLC. The DO and pH of the solution were checked at 0, 48, and 96 h. The number of dead individuals was counted at 96 h and the median lethal concentration (LC50) was determined on the basis of geometrical mean of the measured concentrations of the tested antimicrobials using probit or logit conversion with Ecotox-Statics version 2.6d. The Lethal effect concentration (LC50) value of test chemical in fish in a 96 hr study on the basis of mortality effect was observed to be 0.085 mg/L. Thus based on the LC50 value, chemical consider to be toxic and classified as aquatic acute 1 category as per the CLP classification criteria.

 

First study was supported by the second and third study from peer reviewed journal. The exposed adult fathead minnows (Pimephales promelas) for 48 hours to test chemical were studied. Chemicals were added to the conditioned water after water exchanges (100% static renewal). The second day of exposure, fish were removed from the tanks and placed in beakers that were filled with the tank’s water and with air stones. Then, tanks were emptied, and filled with new conditioned water. Chemicals were added to the conditioned water and fish were placed again in the tanks. This was done in order to maintain constant chemical concentrations in the media during the 48 hours of exposure. Three water samples were collected from each tank in pre-rinsed 40 mL amber glass vials with Teflon septa. The first and second water samples (day 0 and day 1) were taken 5 min after water exchange in order to verify that our stock solution delivered to the renewed tanks provided for similar initial conditions for each 24 h period. The last water sample (day 2, no water exchange) was taken 5 min before fish were removed to estimate the degradation rate using a firstorder decay. All water samples were stored at 4C for later chemical analyses by the USEPA Region 8 Laboratory. Samples arrived at the USEPA within 72 h and upon arrival were stored at -20°C until they were extracted at 96 h. The No Observed effect concentration (NOEC) value of test chemical in fish in a 48 hr study on the basis of mortality effect was determine to be 0.0014 mg/L.

 

 

96 hr short term toxicity study was conducted to determine the lethal effect of test chemical in Oncorhynchus mykiss. The Lethal effect concentration (LC50) value of test chemical in Oncorhynchus mykiss in a 96 hr study on the basis of mortality effect was determine to be 0.18 mg/L. Thus based on the LC50 value, chemical consider to be toxic and consider to be classified as aquatic acute 1 as per the CLP classification criteria.

 

In the fourth study from authoritative database short term toxicity of test chemical were studied on fish Ictalurus punctatus. Fish exposed with the chemical for 48 hrs and performed under the static system. Based on the mortality of test fish Ictalurus punctatus by the chemical after the exposure period of 48 hrs, the LC50 was determine to be 0.14 mg/l. Thus based on the LC50 value, it is concluded that the substance will exhibit toxicity to fish and classified in aquatic acute 1 category.

 

 

Similar study was conducted to determine the effect of test chemical on fish Pimephales promelas. After the exposure period of 96 hr study of test chemical on Pimephales promelas, no effect were observed (NOEC) at 0.054 mg/l on basis of effect on behavior of fish.

 

Thus based on the overall studies, chemical consider to be toxic and classified in aquatic acute 1 category as per the CLP classification criteria.