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EC number: 202-924-1 | CAS number: 101-20-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Density
- Particle size distribution (Granulometry)
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- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Nanomaterial porosity
- Nanomaterial pour density
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- Nanomaterial radical formation potential
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Specific investigations
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- Additional toxicological data

Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: data is from authoritative database
- Justification for type of information:
- data is from authoritative database
- Qualifier:
- according to guideline
- Guideline:
- other: refer principle below
- Qualifier:
- according to guideline
- Guideline:
- other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"
- Principles of method if other than guideline:
- BCF value of test chemical is determined in fish Cyprinus carpio for 28 days on the basis of lipid content
- GLP compliance:
- not specified
- Radiolabelling:
- not specified
- Details on sampling:
- 4 d 7 d 14 d 21 d 28 d (d= days)
- Vehicle:
- yes
- Remarks:
- Name and concentration of vehicle : HCO-40,
- Details on preparation of test solutions, spiked fish food or sediment:
- PREPARATION AND APPLICATION OF TEST SOLUTION
Concentration of vehicle: 1st Concentration area: HCO-40 2 mg/L, Acetone 1.6 mg/L, 2nd Concentration area: HCO-40 2 mg/L, Acetone 1.6 mg - Test organisms (species):
- Cyprinus carpio
- Route of exposure:
- aqueous
- Test type:
- not specified
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 8 wk
- Details on test conditions:
- RANGE-FINDING / PRELIMINARY STUDY - Test concentrations : LC50(96h) > 5.0 mg/L, Rice fish (Oryzias latipes)
- Nominal and measured concentrations:
- - Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/L
- Lipid content:
- 3.6 %
- Time point:
- start of exposure
- Lipid content:
- 3.4 %
- Time point:
- end of exposure
- Conc. / dose:
- 0.02 mg/L
- Type:
- BCF
- Value:
- >= 63 - <= 93 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Remarks on result:
- other: other details not available
- Conc. / dose:
- 0.002 mg/L
- Type:
- BCF
- Value:
- >= 61 - <= 98 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Remarks on result:
- other: other details not available
- Conc. / dose:
- 0.02 mg/L
- Type:
- BCF
- Value:
- 80 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: other details not available
- Conc. / dose:
- 0.002 mg/L
- Type:
- BCF
- Value:
- 81 L/kg
- Basis:
- total lipid content
- Time of plateau:
- 28 d
- Calculation basis:
- steady state
- Remarks on result:
- other: other details not available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.
- Executive summary:
Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio.
Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.
Reference
Table 1 Bioaccumulation test results
|
|
4 d
|
7 d |
14 d |
21 d |
28 d |
1st concentration area
|
Test substance concentration (mg/L) |
0.0191 |
0.0185 |
0.0188 |
0.0195 |
0.0188 |
Bioconcentration factor (L/kg)
|
72 |
85 |
63 |
93 |
89 |
|
66 |
83 |
71 |
78 |
84 |
||
2nd concentration area
|
Test substance concentration (mg/L) |
0.00194 |
0.00182 |
0.00187 |
0.00189 |
0.00193 |
Bioconcentration factor (L/kg)
|
74 |
65 |
68 |
97 |
88 |
|
61 |
98 |
67 |
78 |
85 |
Description of key information
Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio. Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.
Key value for chemical safety assessment
- BCF (aquatic species):
- 81 L/kg ww
Additional information
Two experimental studies have been reviewed to determine bioconcentration factor of test chemical from different sources and their results are summarized below.
In first study the bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio. Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.
In another study the Bioconcentration factor of test chemical was determined in Blue gill fish by flow through system for 30 days. Test chemical concentration used in the study was 0.72µg/L. The depuration duration of this study was 8 days. In this study population of approximately 80 bluegill (Lepomis macroiborius), 5 cm length, were exposed to a nominal test chemical concentration of 1.0µg/L.ina flow through system .The test chemical was 3.4 ring labelled HC with a specific activity of 4.54 mCi/mM (Monsanto Code 103). The bluegills were exposed for 30 days of uptake and 8 days of depuration. Fish were sampled on days 2, 7, 14, 22, 24, and 30 of uptake and on days of depuration. On each sampling day some fish were left whole and others were dissected into muscle, gills, intestines, liver, gall bladder, spleen, and remains. Dissected fish were pooled, two fish per observation with two observations in each mean estimate. All fish were dried, weighed combusted to carbon dioxide, and counted for 1C activity. The BCF value was determined to be 887 wt/wt. in whole fish. By considering this BCF value it is concluded that test chemical is non-bioaccumulative in blue gill fish.
By considering results of both the studies mentioned above it is observed that bioaccumulation concentration factor (BCF) in blue gill and cyprinus carpio fish was in range of 61- 887 L/Kg which is less than 2000. On the basis this range BCF value it is concluded that test chemical is non bioaccumulative in nature according to CLP classification criteria.
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