Triclocarban

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: data is from authoritative database

Justification for type of information:

data is from authoritative database

Qualifier:

according to guideline

Guideline:

other: refer principle below

Qualifier:

according to guideline

Guideline:

other: "Bioaccumulation test of a chemical substance in fish or shellfish" provided in "the Notice on the Test Method Concerning New Chemical Substances"

Principles of method if other than guideline:

BCF value of test chemical is determined in fish Cyprinus carpio for 28 days on the basis of lipid content

GLP compliance:

not specified

Radiolabelling:

not specified

Details on sampling:

4 d 7 d 14 d 21 d 28 d (d= days)

Vehicle:

yes

Remarks:

Name and concentration of vehicle : HCO-40,

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION
Concentration of vehicle: 1st Concentration area: HCO-40 2 mg/L, Acetone 1.6 mg/L, 2nd Concentration area: HCO-40 2 mg/L, Acetone 1.6 mg

Test organisms (species):

Cyprinus carpio

Route of exposure:

aqueous

Test type:

not specified

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

8 wk

Details on test conditions:

RANGE-FINDING / PRELIMINARY STUDY - Test concentrations : LC50(96h) > 5.0 mg/L, Rice fish (Oryzias latipes)

Nominal and measured concentrations:

- Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/L

Lipid content:

3.6 %

Time point:

start of exposure

Lipid content:

3.4 %

Time point:

end of exposure

Conc. / dose:

0.02 mg/L

Type:

BCF

Value:

>= 63 - <= 93 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Remarks on result:

other: other details not available

Conc. / dose:

0.002 mg/L

Type:

BCF

Value:

>= 61 - <= 98 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Remarks on result:

other: other details not available

Conc. / dose:

0.02 mg/L

Type:

BCF

Value:

80 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Calculation basis:

steady state

Remarks on result:

other: other details not available

Conc. / dose:

0.002 mg/L

Type:

BCF

Value:

81 L/kg

Basis:

total lipid content

Time of plateau:

28 d

Calculation basis:

steady state

Remarks on result:

other: other details not available

Table 1 Bioaccumulation test results

 

 

		4 d	7 d	14 d	21 d	28 d
1st concentration area	Test substance concentration (mg/L)	0.0191	0.0185	0.0188	0.0195	0.0188
	Bioconcentration factor (L/kg)	72	85	63	93	89
		66	83	71	78	84
2nd concentration area	Test substance concentration (mg/L)	0.00194	0.00182	0.00187	0.00189	0.00193
	Bioconcentration factor (L/kg)	74	65	68	97	88
		61	98	67	78	85

 

Validity criteria fulfilled:

not specified

Conclusions:

The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.

Executive summary:

Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio.

Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in  Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg  and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg  and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during  28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.

Description of key information

Bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio. Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in  Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.

Key value for chemical safety assessment

BCF (aquatic species):

81 L/kg ww

Additional information

Two experimental studies have been reviewed to determine bioconcentration factor of test chemical from different sources and their results are summarized below.

In first study the bioaccumulation test was conducted for 28 days for determination the bioconcentration factor (BCF) of chemical on test organism Cyprinus carpio. Nominal concentrations used in the study are Nominal concentrations 1st Concentration area : 0.020 mg/L, 2nd Concentration area : 0.0020 mg/Land Range finding study was carried out on Rice fish (Oryzias latipes) LC50(96h) > 5.0 mg/L,. The vehicle used in this study was HCO-40.The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio . The lipid concentration was 3.6% at start of exposure and 3.4 % at end of exposure. The bioconcentration factor (BCF) for test chemical was determined in Cyprinus carpio. The BCF value was observed to be in range 63-93 L/kg and 80 L/kg at steady state at dose concentration 0.02 mg/L and in range 61-98 L/kg and 81 L/kg at steady state at dose concentration 0.002 mg/L. On the basis of total lipid content on test organism Cyprinus carpio during 28 days period. Therefore it is concluded that this test chemical is nonbioaccumulative.

In another study the Bioconcentration factor of test chemical was determined in Blue gill fish by flow through system for 30 days. Test chemical concentration used in the study was 0.72µg/L. The depuration duration of this study was 8 days. In this study population of approximately 80 bluegill (Lepomis macroiborius), 5 cm length, were exposed to a nominal test chemical concentration of 1.0µg/L.ina flow through system .The test chemical was 3.4 ring labelled HC with a specific activity of 4.54 mCi/mM (Monsanto Code 103). The bluegills were exposed for 30 days of uptake and 8 days of depuration. Fish were sampled on days 2, 7, 14, 22, 24, and 30 of uptake and on days of depuration. On each sampling day some fish were left whole and others were dissected into muscle, gills, intestines, liver, gall bladder, spleen, and remains. Dissected fish were pooled, two fish per observation with two observations in each mean estimate. All fish were dried, weighed combusted to carbon dioxide, and counted for 1C activity. The BCF value was determined to be 887 wt/wt. in whole fish. By considering this BCF value it is concluded that test chemical is non-bioaccumulative in blue gill fish.

By considering results of both the studies mentioned above it is observed that bioaccumulation concentration factor (BCF) in blue gill and cyprinus carpio fish was in range of 61- 887 L/Kg which is less than 2000. On the basis this range BCF value it is concluded that test chemical is non bioaccumulative in nature according to CLP classification criteria.