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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Data is from experimental study report
Qualifier:
according to guideline
Guideline:
other: EEC, L 251 Vol. 27 (19.09.84) 84/449/EEC,C.5
Deviations:
yes
Remarks:
The volume of the test solution was reduced from 3.01 to 1.51.
Principles of method if other than guideline:
The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. The test substance was added to the medium, homogenized with Nonylphenol 10EO5PO. Instead of 10 and 20 mg/L, 10 and 30 mg/L were tested.
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
other: Bacteria collected from a sewage treatment plant.
Details on inoculum:
Bacteria collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline.
Duration of test (contact time):
28 d
Initial conc.:
10.9 mg/L
Based on:
test mat.
Initial conc.:
31.4 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
Preparation of test material:
1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps.
Vessels : 2 Liter flasks (dark brown glass) equipped with gas inlet and magnetic stirrer.
Water : The test medium was prepared according to the method described in the guideline
Duration : 28 days
Air : = 25 ml/min purified from carbon dioxide.
Reference substance:
aniline
Remarks:
MERCK No. : 1261, Batch : 12/09/89 Stability : 12/03/90 Storage : room temperature; Reference substance : 20 mg/L
Key result
Parameter:
% degradation (CO2 evolution)
Value:
6
Sampling time:
28 d
Remarks on result:
other: 10.9 mg test substance/l
Key result
Parameter:
% degradation (CO2 evolution)
Value:
4
Sampling time:
28 d
Remarks on result:
other: 31.4 mg test substance/l
Details on results:
Theoretical carbon dioxide evolution
Test substance : 10.9 mg/L = 29.83 mg COa/l.S liter,
Test substance : 31.4 mg/L = 86.20 mg COa/l.S liter,
This calculation is based on carbon analysis.
Biodegradation
Test substance : The biodegradation was calculated as :
10.9 mg test substance/l = 6% in 28 days.
31.4 mg test substance/l = 4% in 28 days.
Validity criteria fulfilled:
not specified
Interpretation of results:
not readily biodegradable
Conclusions:
The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.
Executive summary:

28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Description of key information

28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

28-days biodegradation test following the EU Method C.5 (Degradation: Biochemical Oxygen Demand) was performed to determine the ready biodegradability of the test chemical . Bacteria was used as a test inoculum for the study. Bacteria was collected from activated sludge of the sewage treatment plant of CH-4106 Therwil on 16/10/89 The preparation was carried out according to the method described in the guideline. Aniline (20 mg//l) was used as a reference substance for the study. 1200 ml of the mineral solution with the inoculum were aerated for 24 hours in the test vessel. In 300 ml mineral solution 0.5 ml Nonylphenol 10EO5PO (solution of 30 mg in 100 ml bidist. water) and 16.3 rsp. 47.1 mg of test substance were added and homogenized. This solution was given to the test vessel which was immediately connected to the CO2 traps. The CO2 formed by biodegradation was absorbed with NaOH and determined on a carbon analyzer. Due to the poor solubility of the test substance in water, an emulsifier was used to achieve a better distribution in the medium. Determination of the initial CO2 of the 0.05 N sodium hydroxide and the CO2, absorbed in the absorbers filled with 200 ml 0.05 N sodium hydroxide was carried out on the days 3, 7, 10, 13, 17, 20, 24, 27 and 28, respectively. The biodegradation was calculated on the basis of the theoretical carbon content of the test substance and the cumulative quantities of carbon dioxide determined on the days of measurements. The percentage degradation of test substance was determined to be 6.0 and 4.0% (using 10.9 and 30.4 mg/l conc. of test substance) by using CO2 evolution parameter in 28 days. Thus, based on percentage degradation, test chemical is considered to be not readily biodegradable in nature.