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EC number: 800-309-8 | CAS number: 231297-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: acceptable well-documented publication which meets basic scientific principles
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 975
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- GLP compliance:
- no
Test material
- Test material form:
- solid - liquid: suspension
- Details on test material:
- - Name of test material (as cited in study report): Sodium dodecylbenzene sulphonate (DOBC)
- Molecular formula (if other than submission substance): C18H29NaO3S
- Molecular weight (if other than submission substance): 349.48
- Smiles notation (if other than submission substance):CCCCCCCCCCCCC1=CC(=CC=C1)S(=O)(=O)[O-].[Na+]
- InChl (if other than submission substance): 1S/C18H30O3S.Na/c1-2-3-4-5-6-7-8-9-10-11-13-17-14-12-15-18(16-17)22(19,20)21;/h12,14-16H,2-11,13H2,1H3,(H,19,20,21);/q;+1/p-1
- Structural formula attached as image file (if other than submission substance): see Fig.1
- Substance type: organic salt
- Physical state: no data
- Analytical purity:chemically pure determined by thin layer chromatography and isotope dilution analysis.
- Radiochemical purity (if radiolabelling): radiochemically pure determined by thin layer chromatography and isotope dilution analysi
- Specific activity (if radiolabelling): 8.5 µCi/mg
- Locations of the label (if radiolabelling): 1-alkyl position
Constituent 1
- Radiolabelling:
- yes
Test animals
- Species:
- other: dorsal skin (rat) and female abdominal epidermis (human)
- Strain:
- other: Colworth-Wistar rats; human
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 100-120 g
Administration / exposure
- Type of coverage:
- other: in vitro penetration through the skin
- Vehicle:
- other: 25% polyethylene glycol in water or water only
- Duration of exposure:
- 2, 6, 24 hours (rat, human) and 48 hours (human)
- Doses:
- - Nominal doses: DOBS concentration in solutions was 1.2 mg/mL (two test solutions of the [14C] DOBS were used, the first a 3 mM solution in 25% v/v Polyethylene Glycol 400 in water and a second a 3 mM suspension in water);
- Dose volume: 0.25 mL of each test solution was applied to the rat skin (4.9 cm²) and 0.1 mL to the human epidermal samples (0.78 cm²). - No. of animals per group:
- Not relevant
- Control animals:
- no
- Details on study design:
- DOSE PREPARATION
- Method for preparation of dose suspensions: suspensions were prepared by homogenizing and equilibration in an all glass homogenizer.
APPLICATION OF DOSE
VEHICLE
- Concentration (if solution): 25% v/v Polyethylene Glycol 400 in water
ANALYSIS
- Method type(s) for identification: liquid scintillation counting - Details on in vitro test system (if applicable):
- Not reported
Results and discussion
- Signs and symptoms of toxicity:
- not examined
- Dermal irritation:
- not examined
- Absorption in different matrices:
- The results show no measurable penetration (<0.1 µg/cm²) of DOBS through the rat skin up to 24 h after application. DOBC did not penetrate through the human epidermis until 48 hours after application (<0.1 µg/cm²). Permeability constant of 0.1 µcm/min (= 1.0E-7cm/min) was calculated for the isolated human epidermis 6 and 24 hours after skin contact.
- Total recovery:
- - Total recovery (rat skin): 30% [14C] DOBS was recovered in the rinsings and 70% remained associated with the skin of rats;
-Total recovery (human epidermis): 30-50% of [14C] DOBC retained in the human epidermis after rinsing;
- Limit of detection (LOD): 0.1 µg/cm².
Percutaneous absorptionopen allclose all
- Dose:
- 0.1 µg/cm²
- Parameter:
- percentage
- Absorption:
- 0.16 %
- Remarks on result:
- other: 2, 6, 24 hours (rat)
- Remarks:
- based on the applied amount of 0.25 mL DOBC (concentration in vehicle 1.2 mg/mL) to the rat skin of 4.9 cm² and penetration of 0.1 µg/cm² (lower limit of detection was taken as worst-case).
- Dose:
- 0.1 µg/cm²
- Parameter:
- percentage
- Absorption:
- 0.07 %
- Remarks on result:
- other: 2, 6, 24 and 48 hours (human)
- Remarks:
- based on the applied amount of 0.1 mL DOBC (concentration in vehicle 1.2 mg/mL) to the human epidermis of 4.9 cm² and penetration of 0.1 µg/cm² (lower limit of detection was taken as worst-case).
Applicant's summary and conclusion
- Conclusions:
- No significant penetration through the skin can be expected for dodecyl benzene sulphonate.
- Executive summary:
Dodecylbenzene sulphonate (DOBC) was studied in in vitro penetration study through rat skin and human epidermis. Two test solutions of the [14C] DOBS were used, the first a 3 mM solution in 25% v/v Polyethylene Glycol 400 in water and a second a 3 mM suspension in water. 0.25 mL of the test solution was applied to the rat skin (4.9 cm²) and 0.1 mL to the human epidermal samples (0.78 cm²). The concentration of the test material in solutions was 1.2 mg/mL. After 2, 6 and 24 hours (rats) or after 0.5, 1, 2, 3, 4, 6, 7, 8, 24 and 48 hours (human) of exposure, the skin samples were washed with excess of water and the radioactivity was measured in the rinsings and in the tested skin samples to determine the penetration rates. The results show no measurable penetration (<0.1 µg/cm²) of DOBS through the rat skin up to 24 hours. DOBC did not penetrate human epidermis as well (<0.1 µg/cm²). 30% [14C] DOBS was recovered in the rinsings and 70% remained accociated with the skin of rats. 30-50% of [14C] DOBC retained in the human epidermis after rinsing.
To calculate percutaneous absorption rates, penetration of 0.1 µg/cm², the lower limit of detection was taken as worst-case. Based on the applied amount of 0.25 mL to the rat skin (4.9 cm²) and 0.1 mL to the human epidermis (0.78 cm²) and taken into account concentration of DOBC in vehicle of 1.2 mg/mL, 0.16% and 0.065% absorption through the skin was calculated for the rat skin and human epidermis, respectively.
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