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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non GLP, near guideline study, available as unpublished report, restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982
Report Date:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 403 (Acute Inhalation Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): cyclohexane
No further details reported

Test animals

Species:
rat
Strain:
not specified
Sex:
male/female
Details on test animals and environmental conditions:
No details reported

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: air
Details on inhalation exposure:
No details reported
Analytical verification of test atmosphere concentrations:
yes
Duration of exposure:
4 h
Concentrations:
Nominal concentration 32.88 mg/L (9570.43 ppm)
Mean analytical concentration 21250.0±925.82 ppm
No. of animals per sex per dose:
5
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observations recorded once per hour during exposure and once per day thereafter. Bodyweights recorded pre-exposure and on days 2, 3, 4, 7 and 14 post-exposure.
- Necropsy of survivors performed: yes
Statistics:
Not required, limit dose

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LC50
Effect level:
> 32 880 mg/m³ air
Exp. duration:
4 h
Remarks on result:
other: no mortalities
Mortality:
No mortalities
Clinical signs:
other: During exposure, observations in males and females included tremors, hyperactivity, rapid respiration and lying prostrate in cage. During the post-exposure period all females appeared normal, one male had localised alopecia up to day 10 and sores around t
Body weight:
No exposure-related effects
Gross pathology:
No exposure-related effects

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
The acute inhalation LC50 of cyclohexane to male and female rats, following a 4 hour exposure, was >32.88 mg/L.
Executive summary:

The acute inhalation LC50 of cyclohexane to male and female rats, following a 4 hour exposure, was >32.88 mg/L.