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Diss Factsheets

Ecotoxicological information

Toxicity to other above-ground organisms

Administrative data

toxicity to other above-ground organisms
Type of information:
experimental study
Adequacy of study:
supporting study
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study

Data source

Reference Type:
study report

Materials and methods

Principles of method if other than guideline:
Method: other: Pesticide Assessment Guidelines. FIFRA Subdivision L, Hazard Evaluation: Insecticide and Rodenticide Act, Non-Target Insects.Environmental Protection Agency,Office of Pesticide Programs. October,1982.
GLP compliance:

Test material

Constituent 1
Reference substance name:
L-(+)-lactic acid
EC Number:
EC Name:
L-(+)-lactic acid
Cas Number:
2-hydroxypropanoic acid
Details on test material:

Test substrate

Details on preparation and application of test substrate:
Vehicle: water

Test organisms

Test organisms (species):
other: Apis mellifera (honey bee)
Details on test organisms:
Apparently healthy, worker bees were collected from one active hive
located on the Wildlife International Ltd. site. All test bees were less than three weeks of age. Worker bees of the proper age were selected by splitting the hive and moving a portion away from the main colony. Foragingbeeswereallowedtoreturntothemaincolonywhile nurse workers of the desired age remained with the moved portion. The hive was split one day prior to test initiation. All hives are main- tained according to "Bee Keeping in Maryland," Cooperative Extension Services, University of Maryland, Bulletin 223, 1977.

Study design

Study type:
laboratory study
single dosing
Post exposure observation period:
48 hours

Test conditions

Nominal and measured concentrations:
TESTLEVELS: 6.9, 12.8, 25.1, 50.2, and 100.4 µg/bee

Results and discussion

Effect concentrations
48 h
Dose descriptor:
Effect conc.:
> 100.4 other: µg/bee
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
other: dermal contact toxicity (LD50)

Applicant's summary and conclusion

Validity criteria fulfilled:
The contact LD50 value of SY-83 in the honey bee was determined to be greater than 100.4 ug/bee, the highest concentration tested.
Executive summary:

Mortality rates in all five treatment groups were similar to those observed in the control and solvent control groups. Mortalities did not appear to be treatment related.

In conclusion, SY-83 was classified as relatively non-toxic to honey bees. The honey bee, 48 hour contact LD50 value for SY-83, was greater than 100.4 yg/bee, the highest dosage tested