2-Chloro-5-chloromethylpyridine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb 1989

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Version / remarks:

1981

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

454-800-3

EC Name:

-

Cas Number:

70258-18-3

Molecular formula:

C6H5Cl2N

IUPAC Name:

2-chloro-5-(chloromethyl)pyridine

Test material form:

solid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Versuchstierzucht Winkelmann, Borchen, Germany
- Age at study initiation: ca. 2-3 months
- Weight at study initiation: approx. 170-210 g
- Housing: 5 animals per cage
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +/- 2
- Humidity (%): ca. 50
- Air changes (per hr): ca. 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

nose/head only

Vehicle:

other: polyethylene glyocol / ethanol 50/50 (v/v)

Mass median aerodynamic diameter (MMAD):

>= 1.32 - <= 1.51 µm

Geometric standard deviation (GSD):

>= 1.81 - <= 1.98

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

analytical concentrations: 131.8, 896.3, 1404.1 mg/m3 air

No. of animals per sex per dose:

control groups: 10
dose groups: 5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 2 weeks
- Frequency of observations and weighing: clinical signs were examined several times on the day of exposure and at least once daily therafter; body weights were measured before exposure, on days 3 and 7, and weekly thereafter.
- Necropsy of survivors performed: yes
- Other examinations performed: reflex measurements were made on the first post-exposure day.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 1 404 mg/m³ air (analytical)

Based on:

test mat.

Exp. duration:

4 h

Remarks on result:

other: maximum technically attainable concentration

Mortality:

No animals died during exposure and post-observation period up to the maximal technical attainable concentration of 1404 mg/m3.

Clinical signs:

other: At 132 mg/m3 and above all rats showed slightly reddened noses on the exposure day. There were no signs of a concentration-dependent pronounced amplification of this effect.

Body weight:

Body weight development was normal for both sexes.

Gross pathology:

No gross pathological findings were observed.

Other findings:

The reflex measurements carried out on the first post-exposure day did not reveal any treatment-related changes.

Applicant's summary and conclusion

Interpretation of results:

other: no acute hazard potential

Executive summary:

According to OECD TG 403 the acute inhalation toxicity (LC50) is > 1404 mg/m3 air ( maximum technically attainable concentration) for male and female rats. Thus, the test substance revealed no acute hazard potential under the described experimental conditions after a single 4 -hour inhalation exposure to rats.