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EC number: 454-800-3 | CAS number: -
- Life Cycle description
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- Endpoint summary
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- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
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- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
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- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Stock solution: at 0 hours
Control: at 0 and 48 hours
Test concentrations: at 0 and 48 hours - Vehicle:
- no
- Details on test solutions:
- Pretreatment of the test item
A stock solution was prepared to give the desired series of test concentrations. To achieve this 12.8 mg of the test item were added to 1 litre of dilution water and treated for 1 hour in an ultrasonic bath and afterwards stirred for 24 hours on a magnetic stirrer. Finally undissolved particles of the test item were removed by filtration using a folded filter of pore size 7-12 µm. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Name: Daphnia magna STRAUS, parthenogenetic females
- Source: Strain of Bundesgesundheitsamt Berlin
- Maintenance and Acclimatisation: A population of parthenogenetic females of synchronized age structure is maintained since more than 15 years in the test facility under constant temperature conditions (20 +/- 1 °C) at a 16 : 8 hour light-dark photoperiod (illumination: < 1000 lux). The culture water (so-called 'M4 medium') is partly renewed once a week. The Daphnia are exclusively fed with unicellular green algae (Desmodesmus subspicatus) 'ad libitum'. Mortalities of parent Daphnia during the culture period are recorded daily in a semi-quantitative way. The neonates are separated from their parent Daphnia by filtration prior to the acute test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- 14.4 °dH
- Test temperature:
- mean +/- 1 °C (in the temperature range 18-22 °C)
- Nominal and measured concentrations:
- Test concentration/s (nominal): 0.313, 0.625, 1.25, 2.5 and 5 mg/L
Recovery rates ranged from 98.9 - 100% of nominal values at 0 hours, and from 87.5 - 95.8% of nominal values at 48 hours, respectively. - Details on test conditions:
- - Test vessels: 50 mL glass beakers holding 10 neonates in 20 mL of test medium
- Experimental design: 5 test concentrations plus 1 control, 10 neonates per vessel, 2 replicates per concentration/control, no feeding during the exposure period
- static system
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 16 h light : 8 h dark
- Temperature: mean +/- 1 °C (in the temperature range 18-22 °C)
- Aeration: none
- Test concentration/s (nominal): 0.313, 0.625, 1.25, 2.5 and 5 mg/L
- Method of administration: stock solution
- Medium renewal: none
- Duration of exposure: 48 hours
- Criteria of effects: The criterion of adverse effects used in this study was the item-induced alteration of the normal mobility behaviour and the loss of locomotory actions of the neonates, observed at 24 and 48 hours. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- The results are expressed in terms of nominal concentrations at 48h. Recovery rates ranged from 98.9 - 100% of nominal values at 0 hours, and from 87.5 - 95.8% of nominal
values at 48 hours, respectively. - Validity criteria fulfilled:
- yes
- Remarks:
- immobilisation in the controls did not exceed 10% by the end of the test; dissolved oxygen concentration should remained above 3 mg/L throughout the exposure period
- Conclusions:
- An 48h-EC50 of 2.8 mg/L was determined for 2-chloro-5chloromethylpyridine towards daphnids.
- Executive summary:
A study was performed to assess the acute toxicity of 2 -chloro-5 -chloromethylpyridine to Daphnia magna STRAUS under static conditions.
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'.
The Daphnia were exposed to a range of concentrations, nominally 0.313, 0.625, 1.25, 2.5 and 5.0 mg/L of 2-chloro-5 -chloromethylpyridine dissolved in water for 48 h. Ten neonates per vessel and 2 replicates per concentration/control were exposed. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter.
Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: 48h -EC50: 2.8 mg/L.
The results are expressed in terms of nominal concentrations at 24h and 48h. Recovery rates ranged from 98.9 - 100% of nominal values at 0 hours, and from 87.5 - 95.8% of nominal values at 48 hours, respectively.
Reference
Description of key information
A study was performed to assess the acute toxicity of 2 -chloro-5 -chloromethylpyridine to Daphnia magna STRAUS under static conditions.
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Part C, Method 2 'Acute toxicity for Daphnia' which is in most parts equivalent to the OECD Guideline for Testing of Chemicals No. 202 'Daphnia sp., Acute Immobilisation Test and Reproduction Test, Part I - The 24h EC50 Acute Immobilisation Test'.
The Daphnia were exposed to a range of concentrations, nominally 0.313, 0.625, 1.25, 2.5 and 5.0 mg/L of 2-chloro-5 -chloromethylpyridine dissolved in water for 48 h. Ten neonates per vessel and 2 replicates per concentration/control were exposed. Auxiliaries used to prepare the test media were an ultrasonic bath, a magnetic stirrer and a folded filter.
Observations were made on the swimming ability and the immobilisation rate, respectively, after 24 and 48 hours of exposure. The following values were determined: 48h -EC50: 2.8 mg/L.
The results are expressed in terms of nominal concentrations at 24h and 48h. Recovery rates ranged from 98.9 - 100% of nominal values at 0 hours, and from 87.5 - 95.8% of nominal values at 48 hours, respectively.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 2.8 mg/L
Additional information
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