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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
From 07-01-1992 to 22-01-1992
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Remarks:
This study was performed according to DIN 38412 part 27 with GLP statement, but don't give sufficient experimental details. Therefore, this study is considered not assignable.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report date:
1992

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, part 27
Deviations:
not specified
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[3aR-(3aα,5aβ,9aα,9bβ)]-dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan
EC Number:
229-861-2
EC Name:
[3aR-(3aα,5aβ,9aα,9bβ)]-dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan
Cas Number:
6790-58-5
Molecular formula:
C16H28O
IUPAC Name:
(3aR,5aS,9aS,9bR)-3a,6,6,9a-tetramethyl-dodecahydronaphtho[2,1-b]furan
Test material form:
not specified
Details on test material:
- Substance type: clear
- Storage condition of test material: cool and dark until october 1992

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
Not applicable

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances):
- Method: 10 g of the test item was dissolved with 100 mL of deionised water. The test item was stirring for 24 hours with a magnetic stirrer and then, the solution was filtered.
- Eluate: deionised water
- Differential loading: no data
- Control: yes
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): not applicable

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
No data

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
30 min
Remarks on exposure duration:
none
Post exposure observation period:
none

Test conditions

Hardness:
No data
Test temperature:
No data
pH:
No data
Dissolved oxygen:
No data
Salinity:
Not applicable
Nominal and measured concentrations:
loading rate of 10 g/L.
Details on test conditions:
TEST SYSTEM
no data

TEST MEDIUM / WATER PARAMETERS
no data

OTHER TEST CONDITIONS
no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : The respiration rates (oxygen consumption) were measured.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable (limit test)
- Justification for using less concentrations than requested by guideline: not applicable
- Range finding study: no.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
>= 10 g/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
Oxygen consumption for the control (mg/L O2/min) = 2.25; 2.25; 2.2 (mean of 2.23 mg/L O2/min).
Oxygen consumption for the test substance (mg/L O2/min) = 2.7 (0% inhibition)

DOC at the beginning of the test = 13.05 mg/L
DOC at the end of the test = 50.1 mg/L.
Results with reference substance (positive control):
None
Reported statistics and error estimates:
None

Any other information on results incl. tables

No additional information

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
no sufficient information.
Conclusions:
The test item had no inhibitory effect on the respiration rate of Pseudomonas putida after the incubation period of 30 min at the test item concentration of 10 g/L. The 30min-EC0 was at least 10 g/L.
Executive summary:

The inhibitory effect of the test item on the respiration rate of Pseudomonas putida was investigated in a 30 min respiration inhibition test according to the DIN Guideline 38412 part 27 with GLP statement.

A limit test with a nominal test concentration of 10 g/L was performed.

The test item had no inhibitory effect on the respiration rate of Pseudomonas putida after the incubation period of 30 min at the test item concentration of 10 g/L. The 30min-EC0 was at least 10 g/L.