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EC number: 237-149-8 | CAS number: 13669-76-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted according to GLP and OECD Guideline 435
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Borfluorid-Phosphorsäure
- IUPAC Name:
- Borfluorid-Phosphorsäure
- Reference substance name:
- Trihydrogen trifluoro[phosphato(3-)-O]borate(3-)
- EC Number:
- 237-149-8
- EC Name:
- Trihydrogen trifluoro[phosphato(3-)-O]borate(3-)
- Cas Number:
- 13669-76-6
- Molecular formula:
- B F3 O4 P .3 H
- IUPAC Name:
- phosphoric acid; trifluoroborane
- Test material form:
- other: liquid
- Details on test material:
- Purity: Test substance was characterized analytically
Homogeneity: The test substance was homogenous by visual inspection.
Storage stability: The stability under storage conditions over the study period was guaranteed.
pH: ca. 1 (undiluted test substance)
Constituent 1
Constituent 2
Test animals
- Species:
- other: Corrositex® Biobarrier Membrane
- Details on test animals or test system and environmental conditions:
- The Corrositex® assay kit is commercially available from InVitro International. The Corrositex® Biobarrier Membrane is a test system consisting of a reconstituted collagen matrix. The assay is based on the time that is required for the test substance to penetrate through the Corrositex® Biobarrier Membrane and produce a change in the Chemical Detection System (CDS). The Corrositex® assay is used to determine the corrosive potential of test substances. The assay is limited to testing those materials which cause detectable pH changes in the CDS.
Test system
- Duration of treatment / exposure:
- The test substance was applied undiluted.
- Observation period:
- 60 min
- Details on study design:
- The experimental design of this study consisted of a qualification screen with the CDS (to determine if a color change can be detected) and a categorization screen (to categorize weak acids/bases and strong acids/bases), which were performed as a pretest, and a definitive Corrositex® assay. The Corrositex® assay was evaluated on the basis of the color change of the CDS. The time that a color change was observed was recorded manually and the breakthrough times of the four replicates were used to determine the corrosive potential of the test substance.
TEST SUBSTANCE COMPATIBILITY WITH THE ASSAY (QUALITIFCATION SCREEN):
For the qualification screen, 150 µL of the test substance was added to the CDS screening tube. If the test substance failed to produce a color change in the CDS within one minute, the test substance could not be analyzed in this system, and no further testing was required.
CATEGORIZATION SCREEN:
The categorization screen was used to assess the appropriate scoring scale for the test substance. The categorization screen was performed by adding 150 µL of test substance to each tube A and B. Each tube was mixed and the resulting color observed. If required, 2 drops of the "confirm" reagent were added to tube B, the tube mixed, and the resulting color observed. The categorization kit and color chart provided by InVitro International were used to determine the category. The test substance was scored as category 1 (high acid/alkaline reserve) or category 2 (low acid/alkaline reserve).
BIOBARRIER PREPARATION:
The vial containing the biobarrier matrix powder was placed in a water bath at 64 – 68ºC. The entire contents of the biobarrier diluent vial was added slowly to the matrix powder. The stir bar rotated slowly enough to avoid foaming of the solution. Two hundred µL of the solubilized matrix was pipetted into each of the membrane discs. The membrane discs were then refrigerated for at least 2 hours at 2 – 8ºC. The biobarriers were wrapped and stored at 2 – 8ºC for a maximum of 7 days. Any remaining matrix solution was stored at 2 – 8ºC for up to 30 days in order to prepare additional biobarrier membrane discs.
CORROSITEX® ASSAY:
Following the acceptance of the positive control the Corrositex® assay was performed for the test substance. Four vials containing the CDS were used for the test substance. In addition, one vial was used for the PC, NC and for the color (blank) control, each. A membrane disc coated with the biobarrier matrix was placed into one vial containing the CDS and 500 µL of the undiluted test substance was added onto the membrane disc. An electronic time clock was started with the application. The vial was observed for three minutes for any change in the CDS. If no color change was observed within three minutes, the remaining membranes were treated with the test substance. An electronic time clock was started with each application. The vials were observed continuously for the first ten minutes. Thereafter the vials were observed for approximately ten minutes around the time points relevant for evaluation or until breakthrough of the test substance occurred. The elapsed time between test-substance application and the first change in the indicator solution (i.e. barrier penetration) was recorded. The positive control vial was prepared as described above and received one pellet of sodium hydroxide on top of the membrane disc. This vial was monitored continuously until breakthrough had occurred. The negative control vial was prepared as described above and received 500 µL of 10% citric acid. This vial was observed for 60 minutes and was evaluated as “non-corrosive” if no reaction had been observed.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: breakthrough time of test substance through biobarrier
- Remarks on result:
- other:
- Remarks:
- Basis: mean. Remarks: The mean breakthrough time through the biobarrier in 3 vials was 1:12 min.. (migrated information)
Any other information on results incl. tables
Table 2. Breakthrough times of the test substance and the PC (positive control) and NC (negative control).
|
Breakthrough Time [min:s] |
||||
Test substance |
Vial 1* |
Vial 2 |
Vial 3 |
Vial 4 |
Mean |
Lactamoil 70% |
1:23 |
1:12 |
1:10 |
1:02 |
1:12 |
PC (sodium hydroxide, solid) |
12:02 |
- |
- |
- |
- |
NC (10% citric acid) |
NB |
- |
- |
- |
- |
NB = no breakthrough within maximum observation period (60 min)
Applicant's summary and conclusion
- Interpretation of results:
- corrosive
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Based on the observed results it was concluded, that Borfluorid-Phosphorsäure shows a corrosive potential in the Corrositex® - Skin Corrosion Test under the test conditions chosen. The mean breakthrough time determined in the in vitro membrane barrier test was 1 minute and 12 seconds. The breakthrough time indicates that the test substance has a corrosive potential and should be assigned to UN GHS skin corrosivity subcategories 1A or UN Transport Packing Group I as specified in OECD TG 435 (adopted 19 July 2006).
- Executive summary:
The potential of Borfluorid-Phosphorsäure to cause dermal corrosion was assessed by a single topical application of 500 µL of the test substance to the Corrositex®Biobarrier Membrane (Corrositex®assay).
The Corrositex®Biobarrier Membrane is the test system consisting of a reconstituted collagen matrix. The assay is based on the time that is required for the test substance to penetrate through the Corrositex®Biobarrier Membrane and produce a change in the Chemical Detection System (CDS).
The qualification screen demonstrated that the test substance is able to react with the CDS and produce a visible color change. Therefore the membrane barrier test method was determined to be suitable for the evaluation of the corrosive potential of the test substance.
A timescale category test was carried out to distinguish between weak and strong acids or bases. The test substance was assigned to timescale category 1 (having a high acid/alkaline reserve). In the main test four Corrositex®Biobarrier Membranes were treated with the undiluted test substance.
The mean breakthrough time of three membranes of the test substance, determined in the actual Corrositex®assay, was 1 minute and 12 seconds.
Based on the observed results it was concluded, that Borfluorid-Phosphorsäure shows a corrosive potential in the Corrositex® - Skin Corrosion Test under the test conditions chosen. The mean breakthrough time determined in the in vitro membrane barrier test was 1 minute and 12 seconds. The breakthrough time indicates that the test substance has a corrosive potential and should be assigned to UN GHS skin corrosivity subcategory 1A or UN Transport Packing Group I as specified in OECD TG 435 (adopted 19 July 2006).
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