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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report Date:
2004

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
White powder

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
The actual exposure concentration of the test product were determined by the analytical laboratory. Duplicate samples of the specially prepared test flasks at each sampling time (24h; 48h and 72H) were stored at -20°C±2.0°C until analyses.

Test solutions

Vehicle:
no
Details on test solutions:
In the stock solution of 46.5 mg/l a white precipitate was found soring in the volumetric flask. In order to verify the actual concentration the stock solution was filtered and the filtrate was analysed at Cheminova. The result of the analysis was 44 mg/l.

Test organisms

Test organisms (species):
Skeletonema costatum
Details on test organisms:
NIVA BAC 1

Study design

Water media type:
saltwater
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
18.5-19.5°C
pH:
8
Salinity:
28 ‰
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol, CAS No. : 591-35-5

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
> 37.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 37.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
18.9 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
biomass
Details on results:
pH in control vessels deviated > 1 pH unit (1.2) but this is assessed not to have influence on the result.
Results with reference substance (positive control):
- Results with reference substance valid: Yes- EC50: 1.06 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
Cell density increased by a factor of more than 16 during test, results for reference substance within acceptable range, 1.0-2.20 mg/l %
Conclusions:
EC50(72 h) of Fluorolactone, CAS No. 187592-70-7 is 18.9 mg/L for Skelotonema costatum.