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Diss Factsheets

Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Sept 1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
adopted 04 Apr 1984 Paris
Deviations:
yes
Remarks:
The test temperature was slightly lower than the range the guideline recommended
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: all concentrations
- Sampling method: Samples for the analysis by HPLC were taken at 0; 24; 48; 72 and 96 hours
Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 2.00 g of the test substance were dissolved in 20 L of tap water in the test aquaria. This solution was constantly stirred for about 24 hours and then filtered.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebrafish
- Source: German bred; Distributor: Westaquarium, Zierfischzüchterei, Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 2.5 cm estimated

ACCLIMATION
- Acclimation period: 4 weeks
- Feeding frequency during acclimation: twice a day
- Health during acclimation (any mortality observed): no mortality was observed
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
19.9 - 20.5 °C
pH:
8.4 - 8.6
Dissolved oxygen:
7.3 - 9.0
Nominal and measured concentrations:
100 mg/L nominal
0.04 mg/L measured
Details on test conditions:
TEST SYSTEM
- Test vessel: 25 L glass aquaria
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline

OTHER TEST CONDITIONS
- Photoperiod: 12 h light/12 h dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Mortality and visual abnormalities were recorded every 24 h.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 0.04 mg/L
Nominal / measured:
meas. (initial)
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: Saturated solution
Sublethal observations / clinical signs:

Table 1: Cumulative mortality

 Nominal concentration of ZK 10882 (in mg/I)    3 hours    6 hours    24 hours    48 hours    72 hours    96 hours  
 (water control) 0 0 0 0 0 0 0
                 
100 0 0 0 0 0 0
Validity criteria fulfilled:
yes
Conclusions:
The LC50/96 hours of ZK 10882 is higher than a saturated solution with a concentration of 0.04 mg/L.
Executive summary:

The purpose of this study was to determine LC50/96 hours of the substance 6 -Chlor-Chlormethldien to the Zebra fish (Brachydanio rerio).

Ten fish were used for the test solution and ten for the contral group. The fish were exposed to one concentration of the test substance and the dilution water for aperiod of 96 hours. The test solution was prepared by suspending 100 mg/L in dilution water. After stirring the suspension was filtered and the filtrate was used as test solution. Mortalities and visual abnormalities were recorded at approximately 3, 6, 24, 48, 72 and 96 hours. Samples for the analysis by HPLC were taken at 0; 24; 48; 72 and 96 hours . The concentrations found in the samples reached only 0.04 ± 0.01 mg/L. This indicates that the test substance is only poorly soluble in water. No mortality was observed in the control as well as in the test concentration during the whole exposure time and no fish showed any abnormal behaviour. Accordingly, the LC50/96 hours of the test substance is higher than a saturated solution with a concentration of 0.04 mg/L.

Description of key information

The LC50 of the substance is reported to be > 0.04 mg/L measured over a period of 96 h with Danio rerio as test organism.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
0.04 mg/L

Additional information

"shold read: > 0.04 mg/L"