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Key value for chemical safety assessment

Additional information

In an Ames test, Reinblau BLW (1,4-bis[(2,6-diethyl-4-methyl- phenyl)amino]anthraquinone (CAS-No. 32724-62-2) was negative.

In all in-vitro genetic toxicity assays (Ames test, CA, V79/HGPRT test), Reinblau RLW 1,4-bis[(2-ethyl-6-methylphenyl)amino] anthraquinone (CAS 41611 -76 -1) as a surrogate for Reinblau BLW was negative.


Justification for selection of genetic toxicity endpoint
The results of the Ames tests, the ichromosome aberration test and the HPRT test were considered

Short description of key information:
A valid Ames test for Reinblau BLW (CAS 32724-62-2) is available. This test was negative with and without metabolic activation.
Due to the close structural similarity of Reinblau RLW (CAS 41611-76-1) and Reinblau BLW (1,4-bis[(2,6-diethyl-4-methyl- phenyl)amino]anthraquinone (CAS-No. 32724-62-2) has an additional ethylgroup in the aromatic side chains in relation to with1,4-bis(2-ethyl-6-methy1anilino) anthraquinone (CAS-No. 41611-76-1), the similar physicochemical and toxicological properties a read-across between the 2 compounds is justified.
1,4-bis[(2-ethyl-6-methylphenyl)amino]anthraquinone, CAS 41611-76-1 was investigated in a Salmonella/microsome tests (Ames tests). Result: negative, no evidence of mutagenic activity was seen (with and without mutagenic activation). Additional, 1,4-bis[(2-ethyl-6-methylphenyl)amino]anthraquinone was evaluated as negative in an in-vitro MNT and also in a V79/HPRT test.

Endpoint Conclusion: No adverse effect observed (negative)

Justification for classification or non-classification

Due to the negative results in all available in-vitro genetic toxicity tests a classification is not justified.