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EC number: 251-178-3 | CAS number: 32724-62-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Valid studies with Reinblau BLW (CAS 32724-62-2) for skin and eye irritation are available. The results were supported by additional skin and eye irritation studies conducted with Reinblau RLW (CAS 41611-76-1).
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: guideline study
- Principles of method if other than guideline:
- 500 µl of Macrolex blau RR fl. was applied on a 2.5 x 2.5 cm² area at the shaved trunk of 3 rabbits and fixed semi-occlusively for 24 h. Reading was carried out 1, 24, 48, 72 h and 8 d the application.
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Type of coverage:
- semiocclusive
- Preparation of test site:
- shaved
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Duration of treatment / exposure:
- 4 hours
- Observation period:
- 8 days
- Number of animals:
- 3 animals
- Irritation parameter:
- primary dermal irritation index (PDII)
- Basis:
- mean
- Time point:
- other: 24, 72 h
- Score:
- 0
- Max. score:
- 8
- Reversibility:
- other: score = 0 at any time point if reading was possible
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
- Executive summary:
500 µl of Macrolex Blau RR fl. was applied on a 2.5 x 2.5 cm² area at the shaved trunk of 3 rabbits and fixed semi-occlusively for 24 h. Reading was carried out 1, 24, 48, 72 h and 8 d after the application.
At 1, 24, 48, 72 h a determination of the erythema score was not possible due to substance induced coloration of the skin. At 8 d the score was 0. Edema score was 0 at any time point (1, 24, 48, 72 h, and 8 d). The primary irritation index is = 0.
The test substance is classified as non-irritant.
Reference
At 1, 24, 48, 72 h a determination of the erythema score was not possible due to substance induced coloration of the skin. At 8 d the score was 0.
Edema score was 0 at any time point (1, 24, 48, 72 h, and 8 d)
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Principles of method if other than guideline:
- 100 µl Macrolex Blau RR fl. was applied into the conjunctival sac of the right eye of 3 rabbits. Reading was carried out 1, 24, 48 and 72 h, and 8 d afterthe application of the test substance.
- GLP compliance:
- not specified
- Species:
- rabbit
- Strain:
- New Zealand White
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Duration of treatment / exposure:
- 8 days
- Observation period (in vivo):
- 8 days
- Number of animals or in vitro replicates:
- 3 rabbits
- Irritation parameter:
- other: primary irritation index
- Basis:
- mean
- Time point:
- other: 24, 48, and 72 h
- Score:
- 0
- Max. score:
- 110
- Reversibility:
- other: not applicable - score = 0 at any time point
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information
- Executive summary:
100 µl Macrolex Blau RR fl. was applied into the conjunctival sac of the right eye of 3 rabbits. Reading was carried out 1, 24, 48 and 72 h, and 8 d afterthe application of the test substance.
Macrolex RR fl. produced a primary irritation value of 0 concerning irritation in mucous membrane on the eye. Thus, the test substance is not irritating to the eyes.
Reference
Macrolex RR fl. produced a primary irritation value of 0 concerning irritation in mucous membrane on the eye
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
In a valid study according OECD 404 500 µl of the test substance formulated in glycerin were placed on a plaster and applied on the skin of 3 rabbits. After 4 hours the plaster was removed and the exposed skin area was cleaned with water. Erythema/scab building and edema were scored according to Draize at 24, 48 and 72 hours. No erythema and no edema was observed. Score = 0 at any time point.
In a valid study according OECD 405 100 µl (ca. 40 mg) test substance was applied into the conjuctival sac of 3 rabbits. The treated eye was washed after 24 hours with physiological saline. Eyes were examinated after application of test substance for 7 days.
Cornea, iris, conjunctiva (erythema and chemosis) were scored according to Draize at 24, 48 and 72 hours. Mean values (24, 48 and 72 h) for cornea score = 0, iris score = 0, conjuctivae score = 0 and chemosis score = 0. The test substance is not irritating.
The results were supported by skin and eye irritation studies on Reinblau RLW (CAS 41611 -76 -1).
500 µl of the test substance Reinblau RLW formulated in glycerin were placed on a plaster and applied on the skin of 3 rabbits. After 4 hours the plaster was removed and the exposed skin area was cleaned with water. Erythema/scab building and edema were scored according to Draize at 24, 48 and 72 hours. No erythema and no edema was observed. Score = 0 at any time point. Macrolex Blau CA 51056 is not irritating to the skin (exposure time = 4 hours).
100 µl (ca. 40 mg) Macrolex Blau CA 51056 = CAS 41611-76-1 was applied into the conjuctival sac of 3 rabbits. The treated eye was washed after 24 hours with physiological saline. Eyes were examinated after application of the test substance for 7 days. Cornea, iris, conjunctiva (erythema and chemosis) were scored according to Draize at 24, 48 and 72 hours. Mean values (24, 48 and 72 h) for cornea score = 0, iris score = 0, conjuctivae score = 0 and chemosis score = 0. The test substance is not irritating.
Justification for selection of skin irritation / corrosion endpoint:
key study used
Justification for selection of eye irritation endpoint:
key study used
Justification for classification or non-classification
Due to the results of the skin irritation/corrosion and eye irration study a classifiacion is not justified.
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