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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Justification for type of information:
Justification Document for the Category of Six Anthraquinone Dyes
LANXESS Deutschland GmbH has registered five mono-constituent anthraquinone dyes of similar chemical structure using a category approach: Solvent Violet 36 (CAS No 82-16-6), Solvent Green 3 (CAS No 128-80-3), Reinblau RLW (CAS No 41611-76-1), Reinblau BLW (CAS No 32724-62-2) and Solvent Green 28 (CAS No 4851-50-7). Additional data were taken from another registered anthraquinone dye, Solvent Blue 104 (CAS No 116-75-6), leading to a category consisting of six members (see attached justification in Category dossier).


Test performance:
EXPOSURE CONDITION:
- test volume: 300 ml
- incubation temperature: 25 +/- 1 °C
- mixing: 1 magnetic stirrer per test vessel
- test apparatus: Closed system oxygen consumption measuring apparatus (OM-2001, Ohkura Electric Co. (ID code: B))

MONITORING DATA (at the end of the test):
pH measurement:
- Apparatus: pH meter 720A, ORlON Research
- At the start and the end of BOD measurement, pH of the test solution was measured
- pH: 7.2 (at the end)
Parameter:
% degradation (O2 consumption)
Value:
0 - 1
Sampling time:
7 d
Parameter:
% degradation (O2 consumption)
Value:
0 - 2
Sampling time:
14 d
Parameter:
% degradation (O2 consumption)
Value:
0 - 3
Sampling time:
21 d
Parameter:
% degradation (O2 consumption)
Value:
0 - 3
Sampling time:
28 d
Remarks on result:
other: pH: 7.2
Details on results:
The rate of degradation was not calculated based on the DOC analysis because the substance is hardly soluble in water.
The residual concentration of the test substance (initial concentration= 100 mg/L) were 96.9 mg/L, 99.6 mg/L and 97.9 mg/L for all three test suspensions. Degradabilities based on the residual concentration of the test substance were calculated to be 2%, 0% (calculated value: -1%) and 1%, respectively.
Results with reference substance:
Aniline showed 73 % degradation after 14 days and 77% after 28 days.

- The reference compound reached the level of ≥ 60 percent for ready biodegradability within 14 days

- At the end of the test, at the plateau, or the end of the 10-d window, biodegradation in parallels with test item did not differ by more than 20 percentage points

- The oxygen uptake of the inoculum blank was ≤ 60 mg/L

- In cases where the degradation was ≤ 60 %, the pH values were in between 6.0 and 8.5 at the end of the test

Validity criteria fulfilled:
yes
Remarks:
(For details: See above "Any other information on results" )
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
From the degradability results determined over a period of 28 days based on the BOD (average 1% degradation) and the residual concentration of Reinblau RLW (average 1 % degradation), it is concluded that the source substance is not readily biodegradable. No transformation product was generated under the conditions of this test. The results refere to the structure related 1,4-bis(2-ethyl-6-methy1anilino) anthraquinone (CAS-No. 41611-76-1) of 1,4-bis[(2,6-diethyl-4-methylphenyl)amino]anthraquinone (CAS-No. 32724-62-2).
Executive summary:

The study was conducted in accordance with Test Method Relating to New Chemical Substances (Kanpogyo No.5, Yakuhatsu No.615, 49 Kikyoku No392, 1974) which is in all essential parts identical with OECD Guideline 301 C to assess the ready biodegradability. A solution of the Reinblau RLW (Source substance) in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations. The source substance showed only 1 % biodegradation after 28 days and therefore has to be classified as "Not Readily Biodegradable".


Based on category approach, Reinblau BLW is classified as "Not Readily Biodegradable".

Description of key information

Read-Across on Reinblau RLW, Key, Rel 1, Method Relating to New Chemical Substances (Kanpogyo No.5, Yakuhatsu No.615, 49 Kikyoku No392, 1974) / similar to OECD 301C; Mitsubishi (2011): 1% of biodegradation after 28 days 

Based on category approach, it is concluded that Reinblau BLW (CAS N° 32724-62-2) is not readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

Category approach: LANXESS Deutschland GmbH has registered five mono-constituent anthraquinone dyes of similar chemical structure using a category approach: Solvent Violet 36 (CAS No 82-16-6), Solvent Green 28 (CAS No 4851-50-7), Reinblau RLW (CAS No 41611-76-1), Reinblau BLW (CAS No 32724-62-2) and Solvent Green 3 (CAS No 128-80-3). Additional data were taken from another registered anthraquinone dye, Solvent Blue 104 (CAS 116 -75 -6), leading to a category consisting of six members (see attached justification in IUCLID chapter 13). The category consists of substances all having the diamino-anthraquinone structure as a common moiety which is linked to phenyl groups via the amino groups. Differences within the category are described by various alkyl groups bound to the phenyl groups. All substances included in the category approach are poorly water soluble with high adsorption coefficient (log Koc>6) and high partition coefficient (logKow>8).


Data available on other members of the category confirmed that substances of the category are not readily biodegradable:

































 



Solvent Violet 36



Solvent Green 3



Reinblau RLW



Reinblau BLW



Solvent Blue 104#



Solvent Green 28



Ready Biodegradation



Not readily biodegradable


 


(Covered by category members: read-across with Reinblau RLW)



Not readily biodegradable


(Covered by category members: read-across with Reinblau RLW)


 



Not readily biodegradable


 


(similar to OECD-TG 301C)


 



Not readily biodegradable


 


(Covered by category members read-across with Reinblau RLW)



n.a.



Not readily biodegradable


 


(EU Method C.4 – similar to OECD-TG 301F)



Inherent Biodegradation



n.a



n.a



n.a



n.a



Not inherently biodegradable


(similar to OECD-TG 302C)


 



Not inherently biodegradable


(OECD-TG 302B)


 


 



# not registered by LANXESS, data consulted on ECHA disseminated website on April, 11 2022