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EC number: 429-240-8 | CAS number: 212652-59-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study initiation date - 18 February 1999; Experiment start date - 24 February 1999; Experiment completion date - 03 March 1999; Study completion date - 24 March 1999.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Identity: FAT 40'574/B
Batch: WP 23/99
Purity: Approx. 75 %
Appearance: Solid, dark-red powder
Storage: At room temperature at about 20 °C
Expiration Date: 08 February 2006 - Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- 1.0 g/500 ml of the test substance in deionized water. 500 mg/l of the reference substance in deionized water.
- Test organisms (species):
- activated sludge, industrial
- Details on inoculum:
- Test system: Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel) collected on February 23, 1999
Sludge pre-treatment: The inoculum was prepared 1 day prior to the experimental start. The freshly collected activated sludge was washed 3 times with tap water. Subsequently the sludge was aerated. A sample of the activated sludge was taken to determine the dry weight of the suspended solids. The final concentration of the inoculum was adjusted to about 5.0 g/l suspended solids. For the storage over night synthetic sewage feed was added depending on the sludge concentration. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.0 - 21.2 °C
- pH:
- 8.2 - 8.5
- Dissolved oxygen:
- 7.4 - 10.0
- Nominal and measured concentrations:
- Nominal: 25.6, 64, 160, 400 and 1000 mg/l of the test substance.
3.2, 10 and 32 mg/l of the reference substance. - Details on test conditions:
- Sludge dry weight: 1.56 g/l suspended solids
Test volume: 100 ml
Test duration: 3 h
Aeration: 0.1 - 0.2 l/min by Pasteur pipette
Estimation technique: O2 - consumption, measured with an oxygen sensitive electrode system: OXI DIGI 530 (SOP: 00 109) - Reference substance (positive control):
- yes
- Remarks:
- 3,5 - Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Results with reference substance (positive control):
- IC50 (3 h) of the reference substance 3,5-Dichlorophenol: 15 mg/l.
- Validity criteria fulfilled:
- yes
- Conclusions:
- An IC50 value for the test substance was found to be greater than 1000 mg/L.
- Executive summary:
The objective of this study was the determination of the test substance concentration, which reduces the respiration activity of activated sludge by 50 % (IC50 value). This study was conducted according to OECD test guideline 209 in a GLP-certified laboratory. Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel) was inoculum used for this study. 3,5 -Dichlorphenol served as positive control. The test substance concentration was 25.6, 64, 160, 400 and 1000 mg/l. The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. Based on the findings of this study, the IC50 value for the test substance was found to be greater than 1000 mg/L.
Reference
Description of key information
The IC50 value for the test substance was found to be greater than 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 1 000 mg/L
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
The objective of this study was the determination of the test substance concentration, which reduces the respiration activity of activated sludge by 50 % (IC50 value). This study was conducted according to OECD test guideline 209 in a GLP-certified laboratory. Activated sludge of a communal sewage treatment plant (ARA-Pro Rheno Basel) was inoculum used for this study. 3,5 -Dichlorphenol served as positive control. The test substance concentration was 25.6, 64, 160, 400 and 1000 mg/l. The respiration rate of an activated sludge fed with a standard amount of synthetic sewage feed was measured after a contact time of 3 hours. Based on the findings of this study, the IC50 value for the test substance was found to be greater than 1000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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