Dodecylbenzenesulphonic acid

Biotransformation and kinetics

Administrative data

Endpoint:

biotransformation and kinetics

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Fish (Cyprinus carpio 34.6 g) were exposed to combinations of LAS, polyoxyethylene and sorbitan monooleate to assess the influence of these substances on the uptake of LAS. Exposure period was 3 h. Exposure concentration was 1 x 10-5 M LAS. LSC was used for tissue specific analysis, HPLC was used for measurement of gill adsorption. Dechlorinated tap water, filtered over active carbon; hardness 63 mg/L CaCO3; water/fish ratio: 0.14 g/L; no feeding during experiment

GLP compliance:

not specified

Type of medium:

aquatic

Test material

Test material information

Constituent 1

Reference substance name:

Sodium dodecylbenzenesulfonate

EC Number:

246-680-4

EC Name:

Sodium dodecylbenzenesulfonate

Cas Number:

25155-30-0

Molecular formula:

C18H29NaO3S

IUPAC Name:

sodium dodecylbenzenesulfonate

Test material form:

solid: compact

Details on test material:

Labelled linear LAS, isomer distribution not specified. (CAS #25155-30-3)

Results and discussion

Any other information on results incl. tables

Concentration factors for specific tissues (measured by LSC) were for blood 4.2, hepatopancreas 4.0, spleen 1.0, kidney 3.3, heart 1.7, brain 0.6, muscles 0.2, gill 11, gall bladder 21. Adsorption of LAS to gills (Cgill/Cwater), measured by HPLC, isomer specific: 2-phenyl 16; 3-phenyl 5.4; 4-phenyl, 2.6; 5- and 6-phenyl 1.9. C12-LAS associated radiolabel is taken up by gills rapidly. It reaches the highest body level in the gall bladder after only three hours. The adsorption to the gills is related to the phenyl-substitution of the alkane. The closer the benzenesulfonate-group is attached to the terminal carbon atom of the alkyl chain, the higher the adsorption to the gills. As the gills are an important organ in the uptake of xenobiotic compounds, it seems reasonable to expect that those isomers which sorb strongly to the gills will also be taken up preferentially.

Applicant's summary and conclusion

Conclusions:

Concentration factors for specific tissues (measured by LSC) were for blood 4.2, hepatopancreas 4.0, spleen 1.0, kidney 3.3, heart 1.7, brain 0.6, muscles 0.2, gill 11, gall bladder 21. Adsorption of LAS to gills (Cgill/Cwater), measured by HPLC, isomer specific: 2-phenyl 16; 3-phenyl 5.4; 4-phenyl,
2.6; 5- and 6-phenyl 1.9. C12-LAS associated radiolabel is taken up by gills rapidly. It reaches the highest body level in the gall bladder after only three hours. The adsorption to the gills is related to the phenyl-substitution of the alkane. The closer the benzenesulfonate-group is attached to the terminal carbon atom of the alkyl chain, the higher the adsorption to the gills. As the gills are an important organ in the uptake of xenobiotic compounds, it seems reasonable to expect that those isomers which sorb strongly to the gills will also be taken up preferentially.