Dodecylbenzenesulphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.

Qualifier:

according to guideline

Guideline:

other: QSAR model

Principles of method if other than guideline:

EPI Suite v 4.0/ECOSARv1.00

GLP compliance:

no

Remarks:

not applicable. QSAR model

Analytical monitoring:

not required

Vehicle:

no

Test organisms (species):

other: Daphnid species

Test type:

other: QSAR model

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

not applicable. QSAR model

Test temperature:

not applicable. QSAR model

pH:

not applicable. QSAR model

Dissolved oxygen:

not applicable. QSAR model

Salinity:

not applicable. QSAR model

Nominal and measured concentrations:

not applicable. QSAR model

Details on test conditions:

100% Benzenesulfonic acid, dodecyl-(CAS No. 27176-87-0)
SMILES : : O=S(=O)(c1ccc(cc1)CCCCCCCCCCCC)O
CHEM : Benzenesulfonic acid, dodecyl-
MOL FOR:C18 H30 O3 S1
MOL WT :326.50
Log Kow:4.78 (KowWin estimate)
Wat Sol:100 mg/L (measured)

Reference substance (positive control):

not required

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

7.599 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 7.599 mg/l therefore that the substance is moderately toxic to Daphnid.

Overview of QSARs estimations on short-term aquatic toxicity of

 Dodecylbenzenesulfonic acid/ Benzenesulfonic acid, dodecyl-to Daphnid     

 

Method	Results	Remarks	Reference
QSAR estimate	Daphnid , 48-hr LC50 =7.599 mg/L (ppm)   Daphnid, 30-day, ChV (chronic value) = 1.369 mg/L (ppm)	Reliable with restrictions, QSAR estimated data	ECOSAR Program (v1.00)

 

Using the EPIWIN ECOSAR QSAR model aLC50 for fresh water Daphnid of 7.599 mg/l was established.

Daphnid , 48-hr LC50 = 7.599 mg/L (ppm)

Daphnid, 30-day, ChV (chronic value) = 1.369 mg/L (ppm)

 

Validity criteria fulfilled:

yes

Remarks:

US EPA accepted QSAR method for chemicals properties assessment.

Conclusions:

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 7.599 mg/l therefore that the substance is moderately toxic to Daphnid.

Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 7.599 mg/l therefore that the substance is moderately toxic to Daphnid.

 Daphnid , 48-hr LC50 = 7.599 mg/L (ppm)

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.

Qualifier:

according to guideline

Guideline:

other: QSAR model

Principles of method if other than guideline:

EPI Suite v 4.0/ECOSARv1.00

GLP compliance:

no

Remarks:

not applicable. QSAR model

Analytical monitoring:

not required

Vehicle:

no

Test organisms (species):

other: Mysid shrimp

Details on test organisms:

Scientific name: Americamysis bahia
Common name: Opossum shrimp, Mysid shrimp, Mysids

Test type:

other: QSAR model

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not applicable. QSAR model

Test temperature:

not applicable. QSAR model

pH:

not applicable. QSAR model

Dissolved oxygen:

not applicable. QSAR model

Salinity:

not applicable. QSAR model

Nominal and measured concentrations:

not applicable. QSAR model

Details on test conditions:

100% Benzenesulfonic acid, dodecyl-(CAS No. 27176-87-0)
SMILES : : O=S(=O)(c1ccc(cc1)CCCCCCCCCCCC)O
CHEM : Benzenesulfonic acid, dodecyl-
MOL FOR:C18 H30 O3 S1
MOL WT :326.50
Log Kow:4.78 (KowWin estimate)
Wat Sol:100 mg/L (measured)

Reference substance (positive control):

not required

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

1.222 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 1.222 mg/l therefore that the substance is moderately toxic toxic to Mysid shrimp.

Overview of QSARs estimations on short-term aquatic toxicity of 

 Dodecylbenzenesulfonic acid/ Benzenesulfonic acid, dodecyl-to Mysid shrimp   

 

Method	Results	Remarks	Reference
QSAR estimate	Mysid Shrimp, 96 -hr, LC50= 1.222 mg/L (ppm) Mysid Shrimp (SW), 30-day, ChV (chronic value) = 0.046 mg/L (ppm)	Reliable with restrictions, QSAR estimated data	ECOSAR Program (v1.00)

     

Mysid Shrimp, 96 -hr, LC50= 1.222 mg/L (ppm)

Mysid Shrimp (SW), 30-day, ChV (chronic value) = 0.046 mg/L (ppm)

 

Validity criteria fulfilled:

yes

Remarks:

US EPA accepted QSAR method for chemicals properties assessment.

Conclusions:

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 1.222 mg/l therefore that the substance is moderately toxic toxic to Mysid shrimp.

Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 1.222 mg/l therefore that the substance is moderately toxic toxic to Mysid shrimp.

 

 

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: DIN 38412 Part 11.

Principles of method if other than guideline:

All parameters were maintained in accordance with acceptable DIN guidelines.

GLP compliance:

not specified

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Hardness:

NR

Test temperature:

20 °C

pH:

8.4

Dissolved oxygen:

9.8 mg/l

Salinity:

NR

Nominal and measured concentrations:

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR

Details on test conditions:

TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR μS/cm
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Executive summary:

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Reference 4

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: Directive 84/449/EEC, C.2 "Acute toxicity for Daphnia"

Principles of method if other than guideline:

River Po waters were collected at the end of the drainage basin, extracted by means of XAD-2 resins and tested for toxicity on aquatic organisms (Daphnia magna and Selenastrum capricornutum) and for mutagenicity with a modified Ames test. The extracts were analyzed by HRGC/FID and selective detectors for the determination of the most common classes of toxic compounds.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

NR

Test temperature:

20 °C

Salinity:

NR

Nominal and measured concentrations:

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR

Details on test conditions:

TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48 hour(s)
STAND DUR. (D): 2 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.88 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Executive summary:

A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Reference 5

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009 1975. Method for acute toxicity tests with fish, macroinvertebrates and amphibians.

Principles of method if other than guideline:

Acute toxicity tests were conducted on high molecular weight LAS, individual pure homologues, non-linear LAS components (dialkyl tetralin or indane sulfonates (DTIS), and model biodegradation intermediates (sulfophenyl undecane, SØU) in order to determine whether biodegradation decreases toxicity. In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three beakers. No food was added for the duration of the test.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Water fleas); <18 hr old

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

open system

Hardness:

250 mg/L

Test temperature:

not reported

pH:

not reported

Details on test conditions:

This test were performed in 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness. Ten daphnia (<18 hr old) were placed in each of 3 replicate beakers.No food was added.

Reference substance (positive control):

not required

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

10.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: C12 -LAS

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

3.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: C12 -LAS

Details on results:

A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

 Results:The following table shows the acute toxicity of original materials (LC50, mg/L)..

Daphnia magna
	Average chain length	24 hour	48 hour
High molecular weight LAS	13.3	2.6 ± 0.1	2.3 ± 0.1
Individual homologues LAS
C10	10	53.1 ± 0.4	12.3 ± 2.6
C11	11	15.8 ± 3.0	5.7 ± 0.6
C12	12	10.7 ± 1.6	3.5 ± 1.0
C13	13	2.7 ± 0.4	2.0 ± 0.3
C14	14	1.2 ± 0.2	0.7 ± 0.2
Nonlinear LAS components (DTIS)
C10	10	106.0 ± 27.0	98.0 ± 21.3
C12	12	55.1 ± 9.1	34.1 ± 5.1
C14	14	12.4 ± 1.4	10.0 ± 1.0
Model biodegradation intermediates
C4 (SØ Butyrate)	4	~12,000	~6,000
C5 (SØ Valerate)	5	~12,000	~5,000
C11 (SØU)	11	355 ± 150*	208 ± 85*

*Subsequent repurification of this sample yielded a product with the same isomeric composition but with LC50values over 1000 mg/L for daphnids (Swisher et al., 1976).

 

Validity criteria fulfilled:

yes

Conclusions:

A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Executive summary:

The toxicity of high molecular weight LAS, individual LAS homologues, and nonlinear LAS components (DTIS) was measured in a series of aquatic toxicity tests with Daphnia magna. Results show that biodegradation of LAS influences the toxicity, with the remaining LAS becoming less toxic. The 48 -hour LC50 of high molecular weight LAS to Daphnia magna is 2.4 mg/L. A 48 h LC50 of 3.5 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Reference 6

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: published data

Adequacy of study:

weight of evidence

Study period:

May 8, 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna, <24 h old.
No.of daphnids: 5/replicate, 4 replicates/treatment.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

hardness 168 mg/l (CaCO3)

Test temperature:

20±2°C

pH:

pH 8.4-8.5;

Dissolved oxygen:

O2 91-97%;

Nominal and measured concentrations:

Concentration Nominal: 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
(no vehicle), untreated control

Details on test conditions:

Test conditions :Static; at 20±2°C in 225 mL crystallising dishes (covered), containing 100 mL of reconstituted water of hardness 168 mg/l (CaCO3) and pH 8.3, 16 h light.
Phys. meas.: At 0 and 48 h in one replicate for all concentrations; overall ranges for pH 8.4-8.5; O2 91-97%; temperature (0, 24 and 48 h) 20-21°C.
Observations: Immobility/mortality at 24 and 48 h.

Reference substance (positive control):

not required

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (95% CI 2.2-2.7 mg/L)

Details on results:

The 48-h EC50 calculated using trimmed SPK was 2.5 mg/L (95% CI 2.2-2.7 mg/L) ⇔ 2.2 mg a.i./L (95% CI 2.0-2.4 mg a.i./L).

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 of 2.5mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Executive summary:

A 48 h EC50 of 2.5 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Reference 7

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: published data

Adequacy of study:

weight of evidence

Study period:

May 8, 2000

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

Species: Daphnia magna, <24 h old.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

20±1 degree C

pH:

pH 7.8-8.0

Dissolved oxygen:

dissolved oxygen 7.7-8.0;

Nominal and measured concentrations:

0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L

Details on test conditions:

Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.

Reference substance (positive control):

not required

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

5.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: (95% 3.5-6.0 mg/L)

Details on results:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Executive summary:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Reference 8

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: published data

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

The effects of four concentrations of dodecyl benzene sulfonate at four salinity levels on adult Tisbe bulbisetosa were studied in laboratory experiments. The DBS solutions were analysed by the methylene method to control their stability with time.
The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.

GLP compliance:

not specified

Analytical monitoring:

yes

Vehicle:

no

Details on test solutions:

The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.

Test organisms (species):

other aquatic crustacea: Tisbe bulbisetosa

Details on test organisms:

Tisbe bulbisetosa : adult female

Test type:

not specified

Water media type:

saltwater

Limit test:

no

Total exposure duration:

48 h

Nominal and measured concentrations:

This study is the combined effects of four concentrations (0, 3, 6, 9, 12 ppm) of DBS at four salinity (9, 12, 18, 360) on adult Tisbe bulbisetosa.

Reference substance (positive control):

not required

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

4.58 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

5.12 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)-

Details on results:

- 48 hr-LC50 (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)- 48 hr-LC50 (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.

Reference 9

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

other: published data

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

according to guideline

Guideline:

other: EPA-660/3-75-009

Principles of method if other than guideline:

Environmnetal Protection Agency (1975) ; EPA-660/3-75-009, Method for acute toxicity tests with fish, macroinertebrates and amphibians

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

no

Details on test solutions:

In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna <24 hr old

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

165 mg/L (as CaCO3)

Test temperature:

21-23 deg C

pH:

8.1-8.9

Dissolved oxygen:

8.4 ppm

Nominal and measured concentrations:

- five test concentrations and control (nominal)

Details on test conditions:

- Dilution water was a reconstituted water.(average Hardness (as CaCO3): 165 mg/L, Alkalinity (as CaCO3): 120 +/- 10 mg/L, Dissolved oxygen 5.3mg/L (>60% saturation), temperature : 22 +/- 1 deg C, pH : 7.9-8.4)- Number of replicates; 3 replicates- light intensity (12hr photoperiod at 40-70 L/ft^2)- The 250 mL glass test chambers contained 200ml of test water and five test Daphnia.- five concentrations and control

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.8 - 5.6 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: C12 -LAS

Details on results:

A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Validity criteria fulfilled:

yes

Conclusions:

A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Executive summary:

A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Description of key information

A 24 h EC50 of 12 mg/L has been determined for the effects of the Dodecylbenzenesulfonic acid on immobilation of the Daphnia magna.
A 48 h EC50 of 5.88 mg/L has been determined for the effects of the Dodecylbenzenesulfonic acid on immobilation of the Daphnia magna. 
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS  on mortality of the Daphnia magna.
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 7.599 mg/l therefore that the substance is moderately toxic to Daphnid. 
A 48 h LC50 of 4.58 mg/L  (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

3.5 mg/L

Marine water invertebrates

Marine water invertebrates

Effect concentration:

4.58 mg/L

Additional information

A 24 h EC50 of 12 mg/L has been determined for the effects of the Dodecylbenzenesulfonic acid on immobilation of the Daphnia magna.

A 48 h EC50 of 5.88 mg/L has been determined for the effects of the Dodecylbenzenesulfonic acid on immobilation of the Daphnia magna.

A 48 h LC50 of 3.5 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

 

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 7.599 mg/l therefore that the substance is moderately toxic to Daphnid.

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.