2-amino-2-methylpropanol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

no analytical monitoring

Qualifier:

according to guideline

Guideline:

other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates, and Amphibians" (USEPA, 1975

Deviations:

no

Remarks:

Not specified in report

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Deionized water was used in the preparation of all stock solutions.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnids (Daphnia magna) first instar < 24 hours old. The daphnia used in the test were cultured at the testing facilities, and the adult daphnia were fed a suspension of trout chow and alfalfa daily until 24 hours prior to testing.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

225 ppm as CaCO3

Test temperature:

19-20C

pH:

8.1 in the control, 8.3 (100mg/L AMP), 8.9 (320 mg/L AMP), and 9.4 (1000 mg/L AMP)

Dissolved oxygen:

Dissolved oxygen levels ranging from 8.3 mg/L in the control at study start, and levels of 8.5 (control), 8.5 (100mg/L AMP), 8.6 (320 mg/L AMP), and 8.6 mg/L O2 (1000 mg/L AMP) at 48 hours.

Nominal and measured concentrations:

Nominal- 0, 100, 180, 320, 560, and 1000 mg/L AMP-95

Details on test conditions:

Daphnias (Daphnia magna) first instar < 24 hours old. Test conditions were a temperature range of 19-20C, and a photoperiod of 16 hours light/ 8 hour dark photcycle. Test solution (200mL) was placed in 250mL beakers, and ten daphnias were added per test solution.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

193 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 100-320 mg/L

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

240 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 212-272 mg/L

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

180 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

There was no additional data provided on the results of the range-finding study referenced in the methods section.

Reported statistics and error estimates:

A computerized program calculated the LC50 for Daphnia based on the Probit dose-response model.

Not applicable

Validity criteria fulfilled:

not specified

Conclusions:

In Daphnia magna, 48h NOEC and LC50 values were 100 and 193 mg/L, resp, expressed in test material containing 5% water. This corresponds to NOEC and LC50 values of 95 and 183 mg active substance/L, resp. All values are nominal (no analytical follow-up).

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

marine species, no analytical monitoring

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1045 (Penaeid Acute Toxicity Test)

Deviations:

yes

Remarks:

The test species is a brown shrimp from the North Sea. No analytical verification of the test concentrations was made.

Principles of method if other than guideline:

Not applicable

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Each test solution was prepared by introducing the appropriate weight of test material into 10 litres of clean seawater in the exposure vessel.

Test organisms (species):

Crangon crangon

Details on test organisms:

TEST ORGANISM
- Common name: Brown shrimp
- Source: Tor Bay
- Weight at study initiation (mean and range, SD): average weight 1.9g
- Length at study initiation (length definition, mean, range and SD): average length 49.8 mm

ACCLIMATION
- Acclimation period: 5 days on site fed with a specific diet and a minimum of 3 days at the test temperature.

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

No data

Test temperature:

14.7-15.8C

pH:

8.0-10.1

Dissolved oxygen:

7.2-8.4 mg/L

Salinity:

34.86-34.90 o/oo

Nominal and measured concentrations:

Nominal- 0, 56, 100, 320, and 560 mg/L

Details on test conditions:

Concentrations of AMP-95 tested were 0, 56, 100, 320, and 560 mg/L under semi-static conditions for 96 hours. The 18L glass vessel was folled with 10L of the test solution, and 20 brown shrimp were added to each vessel after an on-site acclimation period of 5 days on a specially-prepared diet. Food was withheld during the test. The test solution was comprised of local filtered seawater (pH=8.10-8.16, salinity 34.86-34.90 o/oo), and was maintained within a temperature range of 14.7-15.8C. The solutions were aerated with compressed air and dissolved O2 ranged from 7.2-8.4 mg/L. The pH range during the test was 8.0-10.1. Hardness was not reported.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

179 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 98.2-329 mg/L

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

241 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CI: 17.8-292 mg/L

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

179 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 98.2-329 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

179 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 98.2-329 mg/L

Details on results:

The control mortality was 0% at 96 hours.

Results with reference substance (positive control):

Not applicable

Validity criteria fulfilled:

not specified

Conclusions:

In marine brown shrimp (Crangon crangon), 48h NOEC and LC50 values were 100 and 179 mg/L, resp., expressed in test material containing 5% water. This corresponds to NOEC and LC50 values of 95 and 170 mg active substance/L, resp. All values are nominal (no analytical follow-up).

Description of key information

All available studies evidence negligible acute toxicity to invertebrates. NOEC and LC50 values were:

• 95 and 183 mg/L, resp. over 48h in freshwater species Daphnia magna;


• not determined and 59 mg/L, over 24h in Daphnia magna (unreliable study);


• 95 and 170 mg , resp. over 48h in marine brown shrimp Crangon crangon.

All values are nominal (no analytical follow-up) and expressed as mg active substance/L. Reported nominal LC50/NOECs were converted into active substance equivalents taking into account the water content of the test items. Measured concentrations in two 2014 algae studies demonstrated the stability in both fresh- and saltwater media over 96h.

The data evidence similar sensitivity of freshwater and marine species.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

LC50

Effect concentration:

183 mg/L

Marine water invertebrates

Marine water invertebrates

Dose descriptor:

LC50

Effect concentration:

170 mg/L