2-amino-2-methylpropanol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

no analytical follow-up

Qualifier:

according to guideline

Guideline:

other: Methods of Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians. Stephan, C. E., Chairman. 1975. Committee on Methods for Toxicity Tests with Aquatic Organisms. U.S. EPA, Ecol. Res. Ser. 660/3-75009.

Principles of method if other than guideline:

Not applicable

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable

Analytical monitoring:

no

Details on sampling:

- Concentrations:0, 32, 56, 100, 180, 320 and 560 mg/l, with ten fish per concentration. Before the test concentrations were prepared, the standard was allowed to warm to room temperature (22°C). The test concentrations were obtained by transferring appropriate aliquots of a working solution directly to the test chambers. The working solution was prepared in deionized water,

Vehicle:

no

Details on test solutions:

Prepared in deionized water. Test concentrations were prepared based on the total compound.

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

Bluegill sunfish were obtained from a commercial supplier, and held in culture tanks with a 16-hour light cycle, and acclimated for 14 days prior to exposure to the test material. Fish received a standard commercial fish food until 48 hours prior to testing, at which time feeding was discontinued. The fish had a mean weight of 0.21 grams and a mean standard length of 22 mm.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Post exposure observation period:

Not applicable

Hardness:

45 mg/L as CaCO3
total alkalinity was 25 mg/L CaCO3

Test temperature:

21-23C

pH:

7.0

Dissolved oxygen:

8.8 mg/L

Nominal and measured concentrations:

Dose levels for the definitive assay were 32-560 mg/L in logarithmic series. Concentrations are reported based on nominal dose.

Dose levels for the definitive 96-hour study were set in a 72-hour range-finding test at 1, 10, 100, 560, 1000 mg/L.

Details on test conditions:

The static bioassay was conducted in 5-gallon glass vessels containing 15 liters of reconstituted water (48mg NaHCO3, 30mg CaSO4, 30mg MgSO4, and 2mg KCl). Vessels were kept in a water bath at 21-23C, and the fish were acclimated to the dilution water and test temperature, and fasted for 48 hours prior to testing.

Reference substance (positive control):

yes

Remarks:

Antimycin A

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

190 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 150-250 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality and sublethal effects

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 170-280 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

220 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 170-280 mg/L

Details on results:

The LC50 data was reported as follows:

AMP 24-hour LC50 = 220 (170-280) mg/L
AMP 48-hour LC50 = 220 (170-280) mg/L
AMP 96-hour LC50 = 190 (150-250) mg/L

Loss of equilibrium and surfacing was noted in some fish at 320 mg/L.

Results with reference substance (positive control):

The LC50 data was reported as follows:

Antimycin A 24-hour LC50 = 0.000056 mg/L
Antimycin A 48-hour LC50 = 0.000037 mg/L
Antimycin A 96-hour LC50 = 0.000028 mg/L

Sublethal observations / clinical signs:

The LC50 was determined with the Probit method

Validity criteria fulfilled:

not specified

Remarks:

There is no information on the stability of the test subtance. No mortality was observed in the control.

Conclusions:

In Lepomis macrochirus, 96h NOEC and LC50 values were 100 and 190 mg/L, resp., expressed in test material containing 5% water. This corresponds to NOEC and LC50 values of 95 and 180 mg active substance/L, resp. All values are nominal (no analytical follow-up).

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not applicable

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

no analytical follow-up

Reason / purpose for cross-reference:

reference to same study

Qualifier:

equivalent or similar to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Deviations:

yes

Remarks:

A saltwater species was tested. No analytical verification of the test concentrations

Principles of method if other than guideline:

The test protocols are appended to the report.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

Concentrations were prepared in sea-water.

Test organisms (species):

Pleuronectes platessa

Details on test organisms:

- Length at study initiation (length definition, mean, range and SD): mean length of 63.2mm
- Weight at study initiation (mean and range, SD): mean weight of 4.1g
- Nb of fish per vessel : 20
- Source: Tor Bay

Test type:

semi-static

Water media type:

saltwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

not reported

Test temperature:

Nominal temperature of the test solution was 15C (14.4-15C during the test).

pH:

8.1-10.3

Dissolved oxygen:

7.0-8.2 mg/L

Salinity:

34.76-34.82 o/oo

Nominal and measured concentrations:

Nominal concentrations tested were 100, 320, 560, 1000 mg/L.

Details on test conditions:

In a 96-hour, semi-static seawater test system, the toxicity of AMP-95 to Plaice was studied. Fish (20 per dose level, mean weight of 4.1g and mean length of 63.2mm) were placed in 18L glass vessels containing 10L of the test solution. The pH of the dilution seawater was 8.08-8.14, and salinity was 34.76-34.82 o/oo. Concentrations tested were 100, 320, 560, 1000 mg/L.

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

LC50

Effect conc.:

231 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 138-385 mg/L

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Duration:

72 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

184 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CL: 31-340 mg/L

Key result

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reported statistics and error estimates:

The LC50 were determined based on the Probit model.

Sublethal observations / clinical signs:

Not applicable

Validity criteria fulfilled:

yes

Remarks:

No mortality was observed in the control, the DO ranged between 7.2-8.4 mg/L and the substance is not hydrolysable.

Conclusions:

In marine fish Pleuronectes platessa, 96h NOEC and LC50 values were 100 and 184 mg/L, resp., expressed in test material containing 5% water. This corresponds to NOEC and LC50 values of 95 and 175 mg active substance/L, resp. All values are nominal (no analytical follow-up).

Description of key information

All available studies evidence negligible acute toxicity to fish. NOEC and LC50 values were:

• 95 and 180 mg/L, resp. over 96h in freshwater fish Lepomis macrochirus; 


• 320 and 331 mg/L, resp. over 48h in freshwater fish Leuciscus idus melanotus;


• 95 and 175 mg/L, resp. over 96h in marine fish Pleuronectes platessa.

All values are nominal (no analytical follow-up) and expressed as mg active substance/L. Reported nominal LC50/NOECs were converted into active substance equivalents taking into account the water content of the test items. Measured concentrations in two 2014 algae studies demonstrated the stability in both fresh- and saltwater media over 96h.

The data evidence similar sensitivity of freshwater and marine species.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

180 mg/L

Marine water fish

Marine water fish

Dose descriptor:

LC50

Effect concentration:

175 mg/L