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EC number: 935-756-9 | CAS number: 1344-95-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07/06/2010-11/04/2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- performed under GLP
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Principles of method if other than guideline:
- n.a.
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Landesamt für Umwelt, Wasserwirtschaft und Gewerbeaufsicht, Rheinland Pfalz. 01/11/2007
Test material
- Reference substance name:
- crystalline calcium silicate hydrate (xonotlite - tobermorite)
- IUPAC Name:
- crystalline calcium silicate hydrate (xonotlite - tobermorite)
- Reference substance name:
- calcium oxosilanebis(olate)
- EC Number:
- 935-756-9
- Cas Number:
- 1344-95-2
- Molecular formula:
- Xonotlite Ca6Si6O17(OH)2 Tobermorite Ca5(Si6O16)(OH)2.4H2O
- IUPAC Name:
- calcium oxosilanebis(olate)
- Reference substance name:
- Amorphous silicic acid, calcium salt
- Molecular formula:
- CaSiO3.nH2O
- IUPAC Name:
- Amorphous silicic acid, calcium salt
- Details on test material:
- - Name of test material (as cited in study report): crystalline Silicic acid, calcium salt (xonotlite - tobermorite)
- Molecular weight (if other than submission substance): unknown
- Physical state: white solid
- Analytical purity: confidential information
- Composition of test material, percentage of components:crystalline calcium silicate hydrate
- Lot/batch No.: confidential information
- Stability under test conditions: in H2O: 96h
- Storage condition of test material: 20 +/- 5°C
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
none
Sampling and analysis
- Analytical monitoring:
- not required
- Details on sampling:
- n.a.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item directly added to solution containing inoculum
- Controls: one positive control; two negative controls; control measurements: 2 replicates before and 2 after measuring positive control and test
item, resp.
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: inoculum was taken from the activation basin of the sewage treatments plant D-67480 Edenkoben, the same day as the experiment was performed. In the test facility the sludge was washed with tap water 3 times, then centrifuged and re-suspended in tap water. Dry matter of sludge: 3.46 g suspended solids/L; dry matter in test: 1.73 g suspended solids/L; pH 7.5
Nutrient solution composition ("synthetic sewage"):
Peptone: 16.0g; Meat extract: 11.0g; Urea: 3.0g; NaCl 0.7g; CaCl2*H2O: 0.4g; MgSO4*7H2O: 0.2g; K2HPO4: 2.8g, in 1000ml deionized water
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Post exposure observation period:
- 5 minutes
Test conditions
- Hardness:
- 0.91 mmol/L
- Test temperature:
- 19.8 - 21.1 °C
- pH:
- 7.5
- Dissolved oxygen:
- test parameter
- Salinity:
- no data
- Nominal and measured concentrations:
- test item nominal: 1000mg/L; positive control substance 3,5-dichlorophenol: 5, 10 , 20, 40 mg/L nominal
- Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers as test vessels; narrow-neck glass bottles as measuring flasks.
- Aeration: purified air, using Pasteur pipettes; flow approx. 0.75 L/min.
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): one (positive control); 2 x 2 negative controls
- loading rate: dry matter in test: 1.73 g suspended solids/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: tap water
- Total organic carbon:< 1.0 mg/L
- Particulate matter: 0
- Metals: Above detection limit: 0.018 mg/L
- Pesticides: < LOD
- Chloride: 9.0 mg/L
- Ca/mg ratio: 16/1
- Conductivity: 189 nS/cm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : O2 content; intervals: 5 mins
TEST CONCENTRATIONS
- Test concentrations:
-positive control: 5, 10, 20, 40 mg/L
- test substance: 1000mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3.5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50: 9.3mg/L, 95% C.I. 7.6-11 mg/L. The value lies within the recommended range of 5- 30mg/L
- Other: R-square (COD), of log/probit regression = 0.998 - Reported statistics and error estimates:
- The maximum variation in the control was 19.2 %;
Any other information on results incl. tables
The measured inhibition rates were the following:
Positive control Inhibition Treatment Inhibition
mg/L % mg/L %
5 33.9 1000 -2.9
10 51.7 1000 3.2
20 71.2
40 82.3
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Relevant validity criteria fulfilled
- Conclusions:
- The 3h-EC50 and 3h-NOEC of Crystalline calcium silicate hydrates on the inhibition of the respiration of activated sludge were > 1000mg/L and ≥ 1000 mg/L ( nominal concentrations) respectively.
- Executive summary:
The inhibition of the respiration of acitvated sludge was evaluated according to OECD TG 209 or EU C.11 (Muckle 2010). The study was performed as a limit test using one concentration in duplicate (1000mg/L). Due to the low water solubility the test item was added to the test vessels directly. The dry matter of the activated sludge was determined as 3.46 g suspended solids/L, giving a concentration of 1.73 g suspended solids/L in the test. The EC50 of the positive control was determined as 9.3 mg/l (95 % C.I. 7.6 -11 mg/L), which lies within the range of 5 -30 mg/L.
The following results for the test item crystalline silicate hydrates (xonolite-tobermorite) were determined: 3h NOEC ≥ 1000 mg/L nominal; 3h EC20 >1000 mg/L nominal; EC50 >1000 mg/L nominal.
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