2-methylbut-3-en-2-ol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1972

Report date:

1972

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

dose volume per kg body weight exceeded the value of 20 mL recommended in the guideline for aqueous suspensions in the highest treatment; no weighing after administration; observation period 7 d

GLP compliance:

no

Remarks:

GLP was not compulsory at the time the study was conducted

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2-methylbut-3-en-2-ol

EC Number:

204-068-4

EC Name:

2-methylbut-3-en-2-ol

Cas Number:

115-18-4

Molecular formula:

C5H10O

IUPAC Name:

2-methylbut-3-en-2-ol

Details on test material:

- Name of test material (as cited in study report): 2-methylbut-3-en-2-ol
- Analytical purity: 98.5-99%
- Physical state: clear liquid

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Gassner (breeder)
- Weight at study initiation: mean 248 g (males), 178 g (females)

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 20%


MAXIMUM DOSE VOLUME APPLIED: 25 mL/kg bw (at 5000 mg/kg bw; amount of applied suspension varied with respective dose groups)

Doses:

1000, 1250, 1600, 2000, 2500, 3200, 4000 and 5000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
- Frequency of observations and weighing: daily observation and several times on the day of application; body weight was determined before the start of the study only
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 1 800 mg/kg bw

Based on:

test mat.

Mortality:

5000 mg/kg bw: all animals died within 24 h, thereof 2 males within 30 min
4000 mg/kg bw: all animals died within 24 h
3200 mg/kg bw: all animals died within 24 h
2500 mg/kg bw: all animals died within 3 d, thereof 4 males and 4 females within 48 h
2000 mg/kg bw: 4/5 males and all females died within 7 d, thereof 3 males and 4 females within 48 h
1600 mg/kg bw: 1/5 male died within 3 d, no female died
1250 mg/ kg bw: 1/5 male died within 48 h and 1/5 females died within 3 d
1000 mg/kg bw: no mortality observed

Clinical signs:

other: Immediately after dosing: tumbling, dyspnea, cyanosis; after 30 min: narcosis-like state, flat respiration, opaque and partially closed eyes, salivation and nose discharge, abdominal position, missing defence reaction; after 1 day: apathy, accelerated res

Gross pathology:

Cardiac dilatation, general congestion, slight gastric dilatation, isolated bloody erosions in the stomach, aqueous diarrheic and sporadic hematolised intestine content.

Any other information on results incl. tables

In a pretest, doses of 200, 1000, 1600 and 5000 mg/kg bw were administered to groups of 5 animals per sex. Here, no animals died in the 200 and 1000 mg/kg bw treatments, respectively, while one male and one female died at 1600 mg/kg bw and all animals died in the 5000 mg/kg bw treatment.