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Toxicity to soil microorganisms

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Description of key information

EC50 > 1000 mg/kg dw soil

Key value for chemical safety assessment

Additional information

One study with 2,2-bis(hydroxymethyl)-1,3-propanediyl dioleate (CAS 25151-96-6) was conducted investigating the effects on nitrogen transformation according to OECD 216 is available (Simon, 2013). In this GLP study nominal test concentrations of 62.5, 125, 250, 500 and 1000 mg/kg dw were tested. Starting at a test substance concentration of 250 mg/kg a decrease of nitrogen transformation was detected. The effect is presumably the result of growth of the microbial community due to the availability of the test item as an organic substrate in an easily available form. The results of the microbial respiration measurements suggest the increase in heterotrophic microbial population due to the addition of the test item, an easily available organic substrate, serving as substrate for microbial growth. It is known that the supply of organic carbon represents a growth advantage for heterotrophic microorganisms which on the other side decrease nitrification rate of the autotrophic nitrifying microorganisms (Strauss & Lamberti, 2000). Consequently, the EC50 for the nitrogen transformation after the test period of 28 days was calculated to be > 1000 mg/kg dw soil. Therefore it can be concluded that the test substance will not exhibit effects to soil microflora.

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