2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(4-ethoxyphenyl)-3-oxobutyramide]

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Year of publication: 1981

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

publication

Title:

THE ASSESSMENT OF THE POSSIBLE INHIBITORY EFFECT OF DYE STUFFS ON AEROBIC WASTE-WATER BACTERIA EXPERIENCE WITH A SCREENING TEST

Author:

Brown, D; Hitz, HR; Schäfer, L

Year:

1981

Bibliographic source:

Chemosphere, Vol. 10, No. 3, pp 245 - 261, 1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

EC Number:

225-822-9

EC Name:

2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]

Cas Number:

5102-83-0

IUPAC Name:

2,2'-[(3,3'-dichlorobiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis[N-(2,4-dimethylphenyl)-3-oxobutanamide]

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

3 h

Test conditions

Test temperature:

20 +- 2°C

pH:

pH: between 7 and 8

Nominal and measured concentrations:

Limit test: 100 mg/L nominal concentration

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Results and discussion

Effect concentrations

Duration:

3 h

Dose descriptor:

IC50

Effect conc.:

> 100 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Remarks:

However, as validity criteria for guideline OECD 209 were specified, these criteria were probably fulfilled for the reported test results.