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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Year of publication: 1981
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
THE ASSESSMENT OF THE POSSIBLE INHIBITORY EFFECT OF DYE STUFFS ON AEROBIC WASTE-WATER BACTERIA EXPERIENCE WITH A SCREENING TEST
Author:
Brown, D; Hitz, HR; Schäfer, L
Year:
1981
Bibliographic source:
Chemosphere, Vol. 10, No. 3, pp 245 - 261, 1981

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]
EC Number:
225-822-9
EC Name:
2,2'-[(3,3'-dichloro[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[N-(2,4-dimethylphenyl)-3-oxobutyramide]
Cas Number:
5102-83-0
IUPAC Name:
2,2'-[(3,3'-dichlorobiphenyl-4,4'-diyl)didiazene-2,1-diyl]bis[N-(2,4-dimethylphenyl)-3-oxobutanamide]

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
not specified

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
3 h

Test conditions

Test temperature:
20 +- 2°C
pH:
pH: between 7 and 8
Nominal and measured concentrations:
Limit test: 100 mg/L nominal concentration
Reference substance (positive control):
yes
Remarks:
3,5-dichlorophenol

Results and discussion

Effect concentrations
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
> 100 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
However, as validity criteria for guideline OECD 209 were specified, these criteria were probably fulfilled for the reported test results.