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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil (if used): About 10 g of a mixture of fine ground quartz sand and 550 mg of test substance were mixed with 740g wet artificial soil for each test container to form a test substance concentration of 1000 mg/kg dry weight.
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Zhuhai Hongming Earthworm Breeding park
- Age at test initiation (mean and range, SD): > 2 months
- Weight at test initiation (mean and range, SD): 552 - 571 mg

ACCLIMATION
- Acclimation period: 14d
- Acclimation conditions (same as test or not): same as test
- Health during acclimation (any mortality observed): no mortality observed
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
18.3 - 21.6 °C
pH:
6.84 - 6.89
Moisture:
25.55 - 29.65%
Details on test conditions:
TEST SYSTEM
- Test container (material, size): 1L glass beaker
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4

OTHER TEST CONDITIONS
- Photoperiod: continuous light
- Light intensity: 554 lux (at the beginning) and 511 lux at the end of the test

EFFECT PARAMETERS MEASURED: Weight, behavior and mortality were recorded after 77 and 14 days exposure time.
Nominal and measured concentrations:
Nominal: 1000 mg/kg dw
Reference substance (positive control):
yes
Remarks:
Cloracetamide
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: none
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: 14d-LC50 was 19.71 mg/kg dry weight, and the 95% confidence Iimit was between 18.14 and 21.42 mg/kg dry weight, which was within the range of 10 - 100 mg/kg dry weight.
Validity criteria fulfilled:
yes
Conclusions:
the substance is not acutely harmful to earthworms.

Description of key information

LC50(14d) > 1000 mg/kg dw (nominal) for Eisenia foetida (OECD 207)

Key value for chemical safety assessment

Additional information

One study investigating the short-term toxicity of Fatty acids, C8-18 and C18-unsatd., reaction products with diethanolamine and propylene oxide (CAS 1000817-22-0) to earthworms is available. The study was performed according to OECD guideline 207 under GLP conditions (Liu 2013). A test substance concentration of 1000 mg/kg dw soil was tested in a limit test using Eisenia foetida as test organism. No mortality was observed in control and treatment throughout the test duration of 14 days. Thus, the 14d-LC50 is determined to be > 1000 mg/kg dw soil based on nominal concentrations.