Registration Dossier
Registration Dossier
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EC number: 208-859-5 | CAS number: 544-10-5
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study, sufficiently documented.
Data source
Reference
- Reference Type:
- publication
- Title:
- Evaluation of 10 aliphatic halogenated hydrocarbons in the mouse bone marrow micronucleus test
- Author:
- Crebelli, R. et al.
- Year:
- 1 999
- Bibliographic source:
- Mutagenesis, 14, 207-215
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 1-chlorohexane
- EC Number:
- 208-859-5
- EC Name:
- 1-chlorohexane
- Cas Number:
- 544-10-5
- Molecular formula:
- C6H13Cl
- IUPAC Name:
- 1-chlorohexane
- Details on test material:
- purity 99 % (from Aldrich)
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- CD-1
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Vehicle:
- Olive oil served as vehicle (at 10 ml/kg)
- Duration of treatment / exposure:
- 24 and 48 h
Doses / concentrations
- Remarks:
- Doses / Concentrations:
530 and 930 mg/kg bw (equivalent to 40 and 70-80 % of the LD50)
Basis:
- No. of animals per sex per dose:
- 5
- Positive control(s):
- Colchicin and mitomycin served as positive control substances.
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
There was no increase in the frequencies of micronuclei at either time point and
dose: 0.3 - 0.6 pro 1000 PCE vs 0.6 pro 1000 cells of the neg. control, while
the positive control substances produced about 10-fold higher levels.
Despite obvious acute animal intoxication, no toxic effect on bone marrow was
demonstrated by the PCE/NCE ratio.
Note: All other halogenated compounds tested failed to induce MN.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative
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