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EC number: 231-824-0 | CAS number: 7757-87-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
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- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
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- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
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- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Biotransformation and kinetics
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Genetic toxicity
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- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- see analogue justification attached to chapter 13
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item was directly added to the test medium and subsequently stirred for 24 hours. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- Fresh samples of activated sludge were taken from a sewage treatment plant (Ruhrverband Kläranlage, Schmallenberg, Germany) mainly fed with municipal wastewater.
- Pretreatment: the sludge was washed with isotonic solution. A stock solution of activated sludge in tap water was prepared with a mixed liquor suspended solids level of 4.6 g dry mass sludge/liter. The sludge stock solution was fed with synthetic sewage feed and was
incubated aerated overnight at 20 ± 2 °C in the dark.
- Initial biomass concentration: 1.5 g/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C.
- pH:
- 8.1 - 8.2
- Nominal and measured concentrations:
- Nominal test substance concentrations: 10, 100, 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask, 1 L
- Material, fill volume: glass, 500 mL
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 6 + 1 abiotic control
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water, free from inhibitory concentrations of toxic substances (e.g. Cu2+ ions)
OTHER TEST CONDITIONS
- Adjustment of pH: yes, buffered to 7.5 ± 0.5 using H2SO4 (50 g/L) solution.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Microbial respiration rate (total oxygen uptake including heterotrophic and nitrification uptake)
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1000 mg/L
- Results used to determine the conditions for the definitive study: EC50 > 1000 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- No inhibition of the respiration rate was observed
- Results with reference substance (positive control):
- EC50: 2 - 25 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- There is no significant inhibition of bacterial respiration by Calcium bis(dihydrogenorthophosphate) up to and including a concentration of 1000 mg/L. The EC50 of the test item is > 1000 mg/L.
Reference
Inhibition (%) of the respiration rate by the test item
Test item concentration |
10 mg/L |
100 mg/L |
1000 mg/L |
Inhibition |
-14.1 % |
-4.4 % |
0.5 % |
Description of key information
Key value for chemical safety assessment
Additional information
Experimental data on the toxicity of trimagnesium bis(orthophosphate) (CAS 7757-87-1) to aquatic microorganisms are not available. The assessment was therefore based on studies conducted with the analogue substance calcium bis(dihydrogenorthophosphate) (CAS 7758-23-8) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006, Annex XI, 1.5.
The effect of calcium bis(dihydrogenorthophosphate) on the respiration of activated sludge was tested in a study following OECD guideline 209. Activated sludge was exposed to nominal substance concentrations of 10, 100 and 1000 mg/L respectively. The oxygen consumption of the activated sludge was observed for 3 hours. An effect on the respiration rate of the activated sludge was not observed. At test termination an EC10> 1000 mg/L (nominal) was determined. Based on the read across data toxic effects of trimagnesium bis(orthophosphate) to aquatic microorganisms are not expected.
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