Oleic acid, compound with (Z)-N-octadec-9-enylpropane-1,3-diamine (2:1)

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012-07-10 to 2012-08-02

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

The study was conducted with river water

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All concentration levels of the test item and the control were analysed at least once within 7 days in the fresh (0 hours) and old media (48 or 72 hours).
- Sampling method: For the longest exposure interval of 72 hours samples were taken from the fresh (0 hours) and old media (72 hours) once within the test period.
For the analyses of the old media, separate replicates without algae and daphnids were prepared and stored under test conditions.
At the end of the study, sorption to the glassware of the test vessels was determined exemplarily in a test vessel, which contained algae and daphnids at a concentration level close to the EC10. The vessel was carefully rinsed with dechlorinated water to remove remaining algae and test medium.
- Sample storage conditions before analysis: All original samples were stored at room temperature until sample preparation, if necessary. Prepared samples were stored at room temperature until analysis.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The stock solutions of 100 – 200 – 400 – 800 - 1600 mg/L were prepared one day before application in glass flasks. The test item was directly weighed out into weighing boats. The weighing boats were put into the glass flasks hanging on a nylon twist. An appropriate volume of demineralized water was added. The pH of the demineralized water was adjusted with 1 M HCl to pH 2 ± 0.2. The actual pH-values were in the range of pH 2.14 to 2.20. Thereafter, the stock solutions were stirred with 1100 rpm for 24 hours at 50 °C.
- Eluate: Natural River Water
- Differential loading: 0.50 - 1.00 - 2.00 - 4.00 - 8.00 mg/L
- Controls: 10 replicates of dilution water without test item.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain/clone: Clone 5
- Justification for species other than prescribed by test guideline: Daphnia magna STRAUS is recommended in the guideline
- Source: Own breeding (Origin: Institut fuer Wasser- Boden- und Lufthygiene)
- Age of parental stock (mean and range, SD): > 14 days
- Feeding during test
- Food type: Mix of Pseudokirchneriella subcapitata and Desmodesmus subspicatus
- Amount: 0.2 mg C/daphnia per day
- Frequency: daily

ACCLIMATION
- Acclimation period: 2 h in dilution water
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: During acclimation the daphnids were not fed
- Feeding frequency: None
- Health during acclimation (any mortality observed): Healthy

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: The parent animals were removed from the culture medium and the juveniles collected over a sieve and flushed into fresh medium

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

Not observed

Hardness:

otal Hardness [mg/L] as CaCO3
Time Weighted Mean
Measured Test item
Concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 19 Day 21
July 11th July 13th July 20th July 23rd July 30th August 1st
6.78 164 151 n.a. n.a. n.a. n.a.
1.74 — — 148 146 n.a. n.a.
0.896 — — — — 173 171
Control 151 148 167 157 167 167

Test temperature:

Temperature [°C]
Time Weighted Mean
Measured Test item
Concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 19 Day 21
July 11th July 13th July 20th July 23rd July 30th August 1st
6.78 22.0 21.2 n.a. n.a. n.a. n.a.
1.74 — — 22.0 21.8 n.a. n.a.
0.896 — — — — 21.8 21.5
Control 21.8 21.1 21.9 21.9 21.6 21.6

pH:

pH-Values
Time Weighted Mean
Measured Test item
Concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 19 Day 21
July 11th July 13th July 20th July 23rd July 30th August 1st
6.78 7.48 7.45 n.a. n.a. n.a. n.a.
1.74 — — 7.56 7.61 n.a. n.a.
0.896 — — — — 7.53 7.57
Control 7.79 7.62 8.00 7.64 8.10 7.53

Dissolved oxygen:

Dissolved Oxygen Concentration [mg/L]
Time Weighted Mean
Measured Test item
Concentration
[mg/L] I F I F I F
Day 0 Day 2 Day 9 Day 12 Day 19 Day 21
July 11th July 13th July 20th July 23rd July 30th August 1st
6.78 8.58 7.45 n.a. n.a. n.a. n.a.
1.74 — — 8.62 8.07 n.a. n.a.
0.896 — — — — 8.52 8.16
Control 8.55 7.77 8.51 8.05 8.72 7.82

Salinity:

Not measured, freshwater

Nominal and measured concentrations:

Please refer to "Any other information on materials and methods"

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: 100 mL glass beaker, filled with 50 mL test solution
- Aeration: No
- Renewal rate of test solution (frequency): 3 x per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control and pH-control (replicates): 10


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
Natural river water of the river Innerste was used. This river is located near 31157 Sarstedt-Ruthe, Germany (52.25°, 9.83°). The natural river water was stored at 15 ± 2 °C until use in the test. The test medium was homogenized by agitation prior to use.
Water Parameters of the Natural River Water
River Innerste
Location Amtshof K514, km 26,
31157 Ruthe-Sarstedt, Germany
Latitude:52.25°, Longitude: 9.83°
Sampling Date 2012-03-20
Weather on Day of Sampling Sunny, slightly clouded; approx. 15°C
Colour Yellowish to brownish, clear
pH 7.93
Conductivity [µS/cm] 856
Dissolved Oxygen [mg O2/L] 11.2
DOC [mg C/L] 2.80*
TOC [mg C/L] 3.66*
Ammonium-N [mg N/L] 0.07
Nitrate-N [mg N/L] 3.40
Total Nitrogen [mg N/L] 3.80
o-Phosphate-p [mg P/L] < 0.20
Total Phosphate [mg P/L] < 0.20
Suspended Matter [mg/L] 14.0
Total Hardness [mg CO3/L] 271
Storage conditions 15 ± 2°C


OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 h light : 8 h dark per day
- Light intensity: Max. 20 µE*m-2*s-1


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Adult mortality: daily
- Number of juveniles: daily
- Stillborn juveniles and aborted eggs: daily
- Appearance of first brood
- Intrinsic rate of natural increase: test end
- Growth (total length and dry weight): test end


RANGE-FINDING STUDY
Immobilization Rates in the Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Immobilization Rates in the Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
Concentration
of the Test Item
[mg/L] IMMOBILIZATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
10 100 100 100 100 100 100
1 0 0 0 0 0 0
0.1 0 0 0 0 0 0
Control 0 0 0 0 0 0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

1.36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

Parent

Remarks on result:

other: No repro tox observed

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

1.41 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks:

parent

Remarks on result:

other: (1 - 2)

Details on results:

- Mortality of parent animals:
The test item induced biologically significant adult mortality of 100 % in the concentration levels of 2.00 to 8.00 mg/L after 21 days. In the concentration levels 0.500 and 1.00 mg/L and in the control all parental daphnids survived till the end of the test after 21 days.
The EC10-value for the adult mortality was estimated by sigmoidal dose-response regression to be 1.36 mg/L. The EC50-value was calculated to be 1.41 mg/L (95 % confidence limits: 1.00 – 2.00 mg/L) as geometric mean of the highest concentration causing 0 % adult mortality (1.00 mg/L) and the lowest concentration causing 100 % adult mortality (2.00 mg/L).
The adult mortality was the most sensitive effect in this study. Therefore, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 1.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 2.00 mg/L.

- No. of offspring produced per day per female:
In the concentration levels of 0.500 and 1.00 mg/L and in the control, 5 broods were produced by the surviving parental daphnids.
The average number of living juveniles per surviving parental daphnids at the end of the test after 21 days was 177 in the control group. No statistically significant reduction of the reproductive output in comparison to the control was determined in the concentration levels of 0.500 and 1.00 mg/L (One Way Analysis of Variance, p = 0.05). An EC10- or EC50-value for the reduction of the reproductive output could not be estimated, because no reduction of the reproductive output > 10 % was observed within the tested concentration levels with surviving daphnids of 0.500 and 1.00 mg/L.
As no statistically significant reduction of the reproductive output was observed in the concentration levels with surviving parental daphnids (0.500 and 1.00 mg/L), the No Observed Effect Concentration (NOEC) after 21 days was assessed at 1.00 mg/L. In consideration of 100 % adult mortality at 2.00 mg/L, the Lowest Observed Effect Concentration (LOEC) was assessed at 2.00 mg/L.
The coefficient of variation of the number of living offspring produced per parent was 6 % in the control. In the concentration levels of 0.500 and 1.00 mg/L with surviving parental daphnids, the coefficients of variation were 8 and 10%, which is regarded to be not biologically significant. For the concentration levels of 2.00 to 8.00 mg/L no coefficients of variation were calculated because of 100 % adult mortality.

- Body length and weight of parent animals:
The mean values of the body length of the surviving parental daphnids in the concentration levels of 0.500 and 1.00 mg/L were 5.88 and 5.90 mm per daphnid. In the control the mean value of the body length was 5.98 mm per daphnid.
The mean dry body weight was in the range of 1.27 and 1.29 mg per daphnid in the concentration levels of 0.500 and 1.00 mg/L and 1.24 mg per daphnid in the control.
- Type and number of morphological / behavioural abnormalities:
No ephippia were observed in the control and in the test groups during the test.
- Time to first brood release or time to hatch:
The first brood was released till day 8 by all parent animals of the control and the concentration levels of 0.500 and 1.00 mg/L.

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC50 (24 h) = 1.79 (CI 1.56 - 2.02) mg/L

Reported statistics and error estimates:

The NOEC and the LOEC based on the adult mortality as the most sensitive effect in this study were determined directly from the observation data.
Significant deviations of the reproduction rates were determined in comparison to the control using statistical standard procedures as normality test, equal variance test and analysis of variance (parametric ANOVA, DUNNETT’S method).
Prior to running the parametric ANOVA, a normality test and an equal variance test were performed. P-values of the normality and equal variance tests were 0.05. The a-value (acceptable probability of incorrectly concluding that there is a difference) was a=0.05.
For the determination of significant deviations of the reproduction rates, One Way Analysis of Variance, DUNNETT’S method (p = 0.05) was used.
The coefficients of variation around the mean number of living offspring produced per parent in the control and the test groups were evaluated.

Mortality [%] of the Adult Daphnids after 7, 14 and 21 Days of Exposure

                 (n = 10)

Nominal Test Item Concentration [mg/L]	Adult Mortality [%]
	7 days	14 days	21 days
8.00	100^1)	100^1)	100^1)
4.00	100^1)	100^1)	100^1)
2.00	0	0	100^1)
1.00	0	0	0
0.500	0	0	0
Control	0	0	0

Number of Juveniles in the Control and Test Groups after 21 Days

Nominal test item Conc. [mg/L]												No. of Parents prod. Juv.	Mean No. of Juveniles per Parent producing Juveniles			Comparison versus Control
	Number of Juveniles in Replicate No.										Total No.				CV
																Inc.	Stat.
	1	2	3	4	5	6	7	8	9	10	å	N	MV ± SD		[%]	[%]
8.00	--	--	--	--	--	--	--	--	--	--	--	0	--		--	--	--
4.00	--	--	--	--	--	--	--	--	--	--	--	0	--		--	--	--
2.00	--	--	--	--	--	--	--	--	--	--	--	0	--		--	--	--
1.00	206	190	212	192	215	193	159	170	171	178	1886	10	189 ±	19	10	7	no
0.500	186	202	191	189	162	198	211	174	202	190	1905	10	191 ±	14	8	8	no
Control	183	174	163	190	161	171	173	180	193	180	1768	10	177 ±	11	6	—	—

Relative Number of Dead (Stillborn + Aborted Eggs) to Total Number of Juveniles

Nominal Test item Concentration [mg/L]	Number of Juveniles			Percentage of Dead Juveniles^#) [%]
	Dead	Alive	Total
8.00	--	--	--	--
4.00	--	--	--	--
2.00	--	--	--	--
1.00	11	1886	1897	1
0.500	5	1905	1910	1
Control	0	1768	1768	0

Total Body Length of the Parental Daphnids

Nominal Test item Concentration [mg/L]	Total Length of the Parent Animals [mm]										MV	N	Dry Weight [mg]
	Replicate No.
	1	2	3	4	5	6	7	8	9	10	[mm]		S	MV
8.00	--	--	--	--	--	--	--	--	--	--	--	0	--	--
4.00	--	--	--	--	--	--	--	--	--	--	--	0	--	--
2.00	--	--	--	--	--	--	--	--	--	--	--	0	--	--
1.00	6.00	5.75	6.00	6.00	5.75	5.75	5.50	6.00	6.00	6.25	5.90	10	12.7	1.27
0.500	6.00	5.75	6.00	5.75	5.50	6.00	6.25	5.50	6.00	6.00	5.88	10	12.9	1.29
Control	6.25	6.00	5.75	5.75	6.25	6.25	6.00	5.50	6.00	6.00	5.98	10	12.4	1.24

Validity criteria fulfilled:

yes

Conclusions:

The overall No Observed Effect Concentration (NOEC) after 21 days was assessed at 0.896 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 1.74 mg/L.
The EC10-value for the adult mortality was estimated by sigmoidal dose-response regression to be 1.11 mg/L. The EC50-value was calculated to be 1.25 mg/L (95 % confidence limits: 0.896 – 1.74 mg/L) as geometric mean of the highest concentration causing 0 % adult mortality (0.896 mg/L) and the lowest concentration causing 100 % adult mortality (1.74 mg/L).

N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminium di[(9Z)-octadec-9-enoate] (CAS No. 34140-91-5)
Endpoints assessed

Time Weighted Mean of the
Measured Concentrations of the Test Item
[mg/L]
EC10 Adult Mortality 1.11 mg/L (95 % confidence limits: Not applicable)
EC50 Adult Mortality 1.25 mg/L (95 % confidence limits: 0.896 – 1.74 mg/L)
EC100 Adult Mortality 1.74 mg/L
NOEC 0.896 mg/L
LOEC 1.74 mg/L

Executive summary:

The Daphnia magna Reproduction Test (semi-static, 21 d) of the test item N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminium di[(9Z)-octadec-9-enoate](CAS No.34140-91-5) was conducted according to OECD 211 (2008) from 2012-07-10 to 2012-08-02, with the definitive exposure phase from 2012-07-11 to 2012-08-01,at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157,.

Test species was Daphnia magna STRAUS (Clone 5). Ten daphnids, individually held, were used per concentration level, and control. At test start the daphnids were 2 to 24 hours old. The study was carried out undersemi-static conditions with renewal of the test solutions three times per week. Aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects or parameters such as time of production of first brood, adult mortality, intrinsic rate of natural increase, occurrence of aborted eggs and stillborn juveniles and body length of the parental daphnids.

Nominal concentrations of N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminiumdi[(9Z)-octadec-9-enoate](CAS No.34140-91-5) were selected as follows:0.500 - 1.00 - 2.00 - 4.00 - 8.00 mg/L.

The tested concentration levels of the test item N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminiumdi[(9Z)-octadec-9-enoate] (CAS No.34140-91-5) and the control were analytically verified by LC-MS/MS of samples on days 0, 9, 19 (fresh media, 0 hours), on days 2, 21 (old media, 48 hours) and on day 12 (old media, 72 hours).

In the fresh media (0 h), the measured concentrations of the test item N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminiumdi[(9Z)-octadec-9-enoate] (CAS No.34140-91-5) were in the range of 88 to 120 % of the nominal values. The recoveries in the old media were in the range of 80 to 89% (48 hours) and 65 to 69 % (72 hours) of the nominal values. All results in this summary given are based on the nominal concentrations of the test item. The time weighted mean measured concentrations were also calculated to be as follows: 0.438 – 0.896 – 1.74 – 3.50 – 6.78 mg/L.
Additionally, the adsorption to the glassware in a test vessel which contained algae and daphnids at the concentration level of 1.00 mg/L was determined to be 4 % of the nominal concentration.

The test item induced biologically significant adult mortality of 100 % in the concentration levels of 2.00 to 8.00 mg/L after 21 days.In the concentration levels 0.500 and 1.00 mg/L and in the control all parental daphnids survived till the end of the test after 21 days.
The 21d-EC10 value for the adult mortality was estimated by sigmoidal dose-response regression to be 1.36 mg/L. The 21d-EC50 value was calculated to be 1.41 mg/L (95 % confidence limits: 1.00 – 2.00 mg/L) as geometric mean of the highest concentration causing 0 % adult mortality (1.00 mg/L) and the lowest concentration causing 100 % adult mortality (2.00 mg/L).

 

The adult mortality was the most sensitive effect in this study. Therefore, the No Observed Effect Concentration (NOEC) after 21 days was assessed at 1.00 mg/L and the Lowest Observed Effect Concentration (LOEC) was assessed at 2.00 mg/L.

 

The average number of living juveniles per surviving parental daphnids at the end of the test after 21 days was 177 in the control group. No statistically significant reduction of the reproductive output in comparison to the control was determined in the concentration levels of 0.500 and 1.00 mg/L (One Way Analysis of Variance, p = 0.05). In the concentration levels of 2.00 to 8.00 mg/L 100 % adult mortality was observed. An EC10 or EC50 value for the reduction of the reproductive output could not be estimated, because no reduction of the reproductive output > 10 % was observed within the tested concentration levels with surviving daphnids of 0.500 and 1.00 mg/L.

 

The coefficient of variation of the number of living offspring produced per parent was 6 % in the control. In the concentration levels of 0.500 and 1.00 mg/L with surviving parental daphnids, the coefficients of variation were 8 and 10%. For the concentration levels of 2.00 to 8.00 mg/L no coefficients of variation were calculated because of 100 % adult mortality.

 

No stillborn juveniles and aborted eggs were produced by the control group during the exposure period of 21 days. Related to the total number of produced juveniles (dead + alive) the percentage of dead juveniles was < 1 % in the concentration levels of 0.500 and 1.00 mg/L.

 

The first brood was released till day 8 by all surviving parental daphnids in the control and the concentration levels of 0.500 and 1.00 mg/L.
In the tested concentration levels of 0.500 and 1.00 mg/Land in the control, 5 broods were produced by the surviving parental daphnids.

 

The intrinsic rates of natural increase (IR) of the surviving parent animals accounting for generation time and number of offspring were used for calculation of population growth and maintenance.

 

The mean values of the body length of the surviving parental daphnids in the concentration levels of 0.500 and 1.00 mg/L were 5.88 and 5.90 mm per daphnid. In the control the mean value of the body length was 5.98 mm per daphnid.

 

The mean dry body weight was in the range of 1.27 and 1.29 mg per daphnid in the concentration levels of 0.500 and 1.00 mg/L and 1.24 mg per daphnid in the control.  No males and ephippia (winter eggs) were observed in the control or in the test groups.

 

A summary of all test item related effects and the assessed endpoints for adult mortality and reproduction is given in Table 1.

 

Table1:       Test Item Related Effects (NOEC, LOEC, EC10, EC50and EC100)

(based on the nominal and the time weighted mean of the measured test item concentrations)

N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminiumdi[(9Z)-octadec-9-enoate](CAS No.34140-91-5)
Effects		Nominal Test Item Concentrations [Time Weighted Mean Measured Concentrations] [mg/L]
		Control	0.500 [0.438]	1.00 [0.896]	2.00 [1.74]	4.00 [3.50]	8.00 [6.78]
Adult Mortality after 21 Days [%]		0	0	0	1001)	1001)	1001)
EC10 Adult Mortality		1.36 mg/L (95 % confidence limits: Not applicable) [1.11 mg/L]
EC50 Adult Mortality		1.41 mg/L (95 % confidence limits: 1.00 – 2.00 mg/L) [1.25 mg/L (95 % confidence limits: 0.896 – 1.74 mg/L)]
EC100 Adult Mortality		2.00 mg/L[1.74 mg/L]
Mean Number of Living Juveniles per Producing Parent (Reproduction Rate±SD)		177±11	191±14	189±19	---	---	---
Coefficient of Variation of theMean Number of Juveniles per Producing Parent [%]		6	8	10	---	---	---
Percentage of Dead Juveniles Related to the Total Number of Juveniles [%]		0	<1	<1	---	---	---
Appearance of First Brood [Mean Day]		8.0	7.9	8.0	---	---	---
Number of Broods		5	5	5	---	---	---
Mean Intrinsic Rates of Natural Increase		0.51	0.51	0.49	---	---	---
Parent Animals	Mean Body Length [mm]	5.98	5.88	5.98	---	---	---
	Mean Dry Weight [mg]	1.24	1.29	1.27	---	---	---
NOEC		1.00 mg/L[0.896 mg/L]
LOEC		2.00 mg/L[1.74 mg/L]

SD   = Standard deviation

1)      = Significant adult mortality (> 20 %)

[…]   = Values in brackets are based on the time weighted mean measured concentrations of the test item.

 

Water quality parametersas pH-value, dissolved oxygen, water hardness and temperature were within the acceptable limits.

 

In order to prove the validity of the test system and test conditions at the test facility, an acute immobilization test according to DIN 38412 L 11 is carried out with potassium dichromate as reference item once per month.
The EC50of the reference item at 1.79 mg/L (95% confidence limits: 1.56 - 2.02 mg/L)after 24 hours is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.

Description of key information

No long-term effects on daphnia reproduction were observed during the chronic daphnia test. For adult mortality an EC10 of 1.35 mg/L in an OECD guideline 211 test in natural river water with Daphnia magna under GLP (reliability 1).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC10

Effect concentration:

1.35 mg/L

Additional information

The Daphnia magna Reproduction Test (semi-static, 21 d) of the test item N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminium di[(9Z)-octadec-9-enoate] was conducted according to OECD 211 under GLP. Nominal concentrations of N-[(9Z)-octadec-9-en-1-yl]propane-1,3-diaminiumdi[(9Z)-octadec-9-enoate] were: 0.500 - 1.00 - 2.00 - 4.00 - 8.00 mg/L. The time weighted mean measured concentrations were: 0.438 – 0.896 – 1.74 – 3.50 – 6.78 mg/L.

The test item did not have any long-term effects on reproduction or development of daphnia. The test substance did however induce biologically significant adult mortality of 100 % in the concentration levels of 2.00 to 8.00 mg/L after 21 days.

21d-EC10 or 21d-EC50 value for the reduction of the reproductive output could not be estimated, because no reduction of the reproductive output > 10 % was observed within the tested concentration levels with surviving daphnids of 0.500 and 1.00 mg/L. The 21d-EC10 and EC50 for adult mortality (the most sensitive endpoint) were found to be 1.35 and 1.4 mg/L respectively, based on nominal concentrations.

 

A Testing Proposal for a long-term toxicity testing on daphnia is submited in order to perfrom the study (according to TG OECD 211) with a standard OECD medium.