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Administrative data

Endpoint:
eye irritation: in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-GLP non-guideline study, available as unpublished report, minor restrictions in design and/or reporting but otherwise adequate for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Principles of method if other than guideline:
HET-CAM in vitro corrosion test (alternative method to study the potential of serious damage to the eyes/mucous membranes in incubated hen eggs).
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Triboron trimethyl hexaoxide
EC Number:
203-016-8
EC Name:
Triboron trimethyl hexaoxide
Cas Number:
102-24-9
Molecular formula:
C3H9B3O6
IUPAC Name:
trimethoxy-1,3,5,2,4,6-trioxatriborinane
Details on test material:
- Name of test material (as cited in study report): Trimethoxyboroxin
- Physical state: liquid
- pH: ca. 5 (undiluted)
- Test substance No.: 04/0710-1
- Lot/batch No.: 559732

Test animals / tissue source

Species:
other: chorionallantoic membrane of fertilized hen eggs

Test system

Vehicle:
unchanged (no vehicle)
Controls:
yes
Amount / concentration applied:
TEST MATERIAL
- Amount applied: 0.3 mL
Duration of treatment / exposure:
single application
Observation period (in vivo):
3.5 minutes
Number of animals or in vitro replicates:
3 eggs
Details on study design:
SCORING SYSTEM:
After application of the test substance the chorionallantoic membrane was observed by means of a stereomicroscope until unambiguous irritation
reactions were detected or up to a maximum time period of 3.5 minutes, respectively.
The time of appearance (in seconds after application) of intravascular resp. extravascular coagulation and, if applicable, other reactions
(haemorrhagia, vessel lysis) were determined.

The evaluation of the reactions was performed according to the following grading:
0 = no visible change
1 = slight reaction
2 = moderate reaction
3 = severe reaction

TOOL USED TO ASSESS SCORE: stereomicroscope

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: time until appearance of coagulation
Basis:
mean
Time point:
other: 65 seconds
Score:
2
Max. score:
3
Irritation parameter:
other: time until appearance of haemorrhagia
Basis:
mean
Remarks on result:
other: not observed within appearance of coagulation
Irritant / corrosive response data:
The chorionallantoic membrane of the eggs treated with the test substance showed moderate (grade 2) intravascular coagulation in all eggs after 49 - 81 seconds (mean: 65 seconds).

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye)
Remarks:
Migrated information

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