3-(5,5,6-trimethylbicyclo[2.2.1]hept-2-yl)cyclohexan-1-ol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of various test chemicals

Justification for type of information:

Data for the target chemical is from collection of data

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: Refer below principle

Principles of method if other than guideline:

WoE report is based on toxicity study of microorganism for the test chemical:
WoE 2 and 3rd

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

other: S. aureus, B. cereus , E. coli , P. aeruginosa

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Details on test conditions:

2nd and 3rd study:

Test vessel: Petri plate
-The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×108 1×106 CFU/ml for bacteria and fungi respectively. Inoculate was wabbed on Muller Hinton Agar.Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

other: MIC

Effect conc.:

> 8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 2nd study

Duration:

48 h

Dose descriptor:

other: MIC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: 3rd study

Validity criteria fulfilled:

no

Conclusions:

The test chemical is likely to be toxic to microorganism atleast in the concentartion range of 1 to 8 mg/l.

Executive summary:

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

In the first study toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be > 8 mg/l.

Above study was supported by the second from secondary source. Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5 McFarland. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l.

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.

Description of key information

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.

Key value for chemical safety assessment

EC50 for microorganisms:

1 mg/L

Additional information

Data available for the test chemicals has been reviewed to determine its effect on microoranism.The studies are as mentioned below:

In the first study toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be > 8 mg/l.

Above study was supported by the second from secondary source. Toxicity of test material was evaluated for microorganisms by disc diffusion method and the minimum inhibition concentration was evaluated using broth dilution method. The organisms used for the test were S. aureus, B. cereus , E. coli , P. aeruginosa. The turbidity of microorganisms was adjusted to 0.5. The inoculum were 1×10⁸1×10⁶CFU/ml for bacteria and fungi respectively. Inoculate was swabbed on Muller Hinton Agar. Sterile blank discs impregnated with 3, 5, 10 μl of oil in 10 μl of dimethyl sulfoxide (DMSO) were used and put on cultured plates. Disc containing DMSO and antibiotics were used as control. The minimum inhibition concentration of test material on bacteria S. aureus, B. cereus , E. coli , P. aeruginosa was observed to be 1 mg/l.

The test chemical is likely to be toxic to microorganism  atleast in the concentartion range of 1 to 8 mg/l.