3-(5,5,6-trimethylbicyclo[2.2.1]hept-2-yl)cyclohexan-1-ol

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Principles of method if other than guideline:

Effect of test chemical was evaluated based on the daphnia magna reproduction.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Test solutions was prepared by dissolving the test substance in the ADAMS medium.

Test organisms (species):

Daphnia magna

Details on test organisms:

- Common name: Water flea.
Strain: straus.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

21±1 °C

Nominal and measured concentrations:

Nominal concentration : 0.25, 0.5, 1, 2, 4 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml Beaker
- fill volume: 50 mL
- Aeration: no aeration
- Renewal rate of test solution (frequency/flow rate): alternate days
- No. of organisms per vessel: 1 daphnia per vessel
- No. of vessels per concentration (replicates): 10 vessels
- No. of vessels per control (replicates): 10 vessels


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2

Reference substance (positive control):

not specified

Key result

Duration:

21 d

Dose descriptor:

EC10

Effect conc.:

0.148 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality: Number of living offspring produced per surviving parental animal {for Daphnia magna, TG 211}

Remarks on result:

other: 95%CI- 0.052-0.419 mg/L

Details on results:

- Behavioural abnormalities:
- Observations on body length:avg parent 0.36cm; Avg offsprings :0.04 cm
- Mortality of control: no mortality was observed in control
- Immobilisation of control: not observed

Validity criteria fulfilled:

yes

Conclusions:

The effect of the test chemical was studied to evaluate its effects on the daphnia magna reproduction and the EC10 value was determined to be 0.148±0.85 mg/L.

Executive summary:

The test substance was used to evaluate its adverse effects to Daphnia magna. The test was conducted in accordance with the OECD 211 guidleines, for the period of 21 days. The test solutions was prepared in the ADAMS medium, the test concentration chosen for the exposure was 0.25 - 4 mg/L, with the geometric series of 2. the test was performed in semi static conditions, wherein the test chemical was renewed every alternate day, of the exposure period. The EC10 was calculated to be 0.148±0.85 mg/L. Based on the Effective concentration it can be classified into chronic 2 category of CLP criteria.

Description of key information

The test substance was used to evaluate its adverse effects to Daphnia magna. The test was conducted in accordance with the OECD 211 guidleines, for the period of 21 days. The test solutions was prepared in the ADAMS medium, the test concentration chosen for the exposure was 0.25 - 4 mg/L, with the geometric series of 2. the test was performed in semi static conditions, wherein the test chemical was renewed every alternate day, of the exposure period. The EC10 was calculated to be 0.148±0.85 mg/L. Based on the Effective concentration it can be classified into chronic 2 category of CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC10

Effect concentration:

0.148 mg/L

Additional information

The test substance was used to evaluate its adverse effects to Daphnia magna. The test was conducted in accordance with the OECD 211 guidleines, for the period of 21 days. The test solutions was prepared in the ADAMS medium, the test concentration chosen for the exposure was 0.25 - 4 mg/L, with the geometric series of 2. the test was performed in semi static conditions, wherein the test chemical was renewed every alternate day, of the exposure period. The EC10 was calculated to be 0.148±0.85 mg/L. Based on the Effective concentration it can be classified into chronic 2 category of CLP criteria.