Registration Dossier
Registration Dossier
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EC number: 203-176-9 | CAS number: 104-12-1
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- GLP compliance:
- yes
- Type of assay:
- micronucleus assay
Test material
- Reference substance name:
- 4-chlorophenyl isocyanate
- EC Number:
- 203-176-9
- EC Name:
- 4-chlorophenyl isocyanate
- Cas Number:
- 104-12-1
- Molecular formula:
- C7H4ClNO
- IUPAC Name:
- 1-chloro-4-isocyanatobenzene
- Details on test material:
- IUCLID4 Test substance: other TS: 4-chlorophenylisocyanate, purity: 99.6%
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- NMRI
- Sex:
- male/female
Administration / exposure
- Route of administration:
- intraperitoneal
- Duration of treatment / exposure:
- single administration
- Frequency of treatment:
- single administration
- Post exposure period:
- animals were sacrificed 16, 24 and 48 hours after the administration of the test substance
Doses / concentrations
- Remarks:
- Doses / Concentrations:
50 mg/kg bw
Basis:
nominal conc.
- No. of animals per sex per dose:
- 10 mice
- Positive control(s):
- positive control: cyclophoshamide; dose: 20 mg/kg bw
Examinations
- Tissues and cell types examined:
- tissue examined: mouse bone marrow
cell types examined: polychromatic to normochromatic erythrocytes, number of polychromatic erythrocytes showing micronuclei
Results and discussion
Test results
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- 1 of 40 animals died before the end ot the test due to the acute ip toxicity
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Any other information on results incl. tables
RS-Freetext:
no indications of a clastogenic effect of p-chlorophenylisocyanate;
the positive control, cyclophosphamide, was functional.
Applicant's summary and conclusion
- Executive summary:
method: micronucleus test on the mouse according OECD 474
result: negative - no indications of a clastogenic effect of the test substance were found after a single intraperitoneal treatment with 50 mg/kg bw
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