Registration Dossier

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Test procedure according to national standards. Study conducted in compliance with GLP regulations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2010
Report date:
2010

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Isopulegol
EC Number:
201-940-6
EC Name:
Isopulegol
Cas Number:
89-79-2
Molecular formula:
C10H18O
IUPAC Name:
isopulegol

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
Test system: Activated sludge from a municipal wastewater treatment plant
Origin of the biological test system: Municipal wastewater treatment plant of Mannheim, Germany
The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with tap water and the suspension was adjusted to 7.5 g/L dry matter.
An aliquot of this supsension was added to the test vessels to obtain a sludge concentratuion of 1.5 g/L dry substance.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
180 min

Test conditions

Nominal and measured concentrations:
1000, 500, 250, 125, 62.5 mg/L and two control replicates

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
EC10
Effect conc.:
430 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
580 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion