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EC number: 700-320-7 | CAS number: 18916-17-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2008-05-15 to 2008-06-12
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP, Guideline study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- , 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- , 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- signed 2007-03-21
Test material
- Reference substance name:
- 1-(4-{[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-{[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}oxan-2-yl]oxy}-2,6-dihydroxyphenyl)-3-(4-hydroxyphenyl)propan-1-one
- EC Number:
- 700-320-7
- Cas Number:
- 18916-17-1
- Molecular formula:
- C27H34O14
- IUPAC Name:
- 1-(4-{[(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-(hydroxymethyl)-3-{[(2S,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}oxan-2-yl]oxy}-2,6-dihydroxyphenyl)-3-(4-hydroxyphenyl)propan-1-one
- Reference substance name:
- 1-[4-[[2-O-(6-Deoxy-L-mannopyranosyl)-D-glucopyranosyl]oxy]-2,6-dihdroxyphenyl]-3(4-hydroxyphenyl)-1-propanone
- IUPAC Name:
- 1-[4-[[2-O-(6-Deoxy-L-mannopyranosyl)-D-glucopyranosyl]oxy]-2,6-dihdroxyphenyl]-3(4-hydroxyphenyl)-1-propanone
- Details on test material:
- - Name of test material (as cited in study report): Naringin dihydrochalcone (DHC); 1-[4-[[2-O-(6-Deoxy-L-mannopyranosyl)-D-glucopyranosyl]oxy]-2,6-dihdroxyphenyl]-3(4-hydroxyphenyl)-1-propanone
- Substance type: solid, whitish powder
- Physical state: technical product
- Storage condition of test material: 15 - 25 °C, dark, dry, don't freeze, keep away from strong light and heat
- no further significante details stated
Constituent 1
Constituent 2
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage treatment plant Ruhrverband Kläranlage, Schmallenberg, Germany (mainly municipal water)
- Method of cultivation: aerobic
- Storage conditions: kept aerobic until use
- Storage length: samples withdrawn on 2008-05-15
- Preparation of inoculum for exposure: wash once with mineral medium after arrival at laboratory
- Concentration of sludge used in the test: 29.6 mg dry mass/litre
- Water filtered: no
- no further significante details stated - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: deionized water; 10 mL/L (a) KH2PO4 (8.50 g/L), K2HPO4 (21.75 g/L), Na2HPO4 x 2 H2O (33.40 g/L), NH4Cl (0.50 g/L); 1 mL/L (b) CaCl2 x 2 H2O (36.40 g/L); 1 mL/L (c) MgSO4 x 7 H2O (22.50 g/L); 1 mL/L (d) FeCl3 x 6 H2O (0.25 g/L)
- Test temperature: 22 +/- 0.1 °C
- pH: 7.4 +/- 0.2
- pH adjusted: no
- Aeration of dilution water: the suspension was stirred during the whole test
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 500 mL glass vessels with a medium volume of 250 mL
- Number of culture flasks/concentration: 2 vessels each for test suspension, inoculum blank, abiotic control, procedural control, toxicity control
- Method used to create aerobic conditions: the suspension was stirred during the whole test
- Measuring equipment: SAPROMAT respirometer (VOITH Inc.) for measuring and recording of oxygen demand
- Test performed in closed vessels due to significant volatility of test substance:
SAMPLING
- Sampling frequency: daily
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control/ procedural control: yes, with reference item
- no further significante details stated
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg/L medium
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- not applicable
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 76
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 64
- Sampling time:
- 13 d
- Remarks on result:
- other: 10d window starting at day 3
- Details on results:
- - see attachmed background material (table 9 of the report)
BOD5 / COD results
- Results with reference substance:
- - 81 % degradation within the first 14 days
Any other information on results incl. tables
The test is considered valid, as
- the difference of extremes of replicate values of the removal of the test item at the end of the 10-day-window and the end of the test was less than 20 %,
- the percentage degradation of the reference item has exceeded the pass level of 60 % by day 14,
- the oxygen uptake of the inoculum blank is < 60 mg/L in 28 days and the pH value was inside the range of 6.0 - 8.5.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see "overall remarks"
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation of Naringin DHC in the static test was found to be 76 % after 28 days. The biodegradation within the 10-day-window was 64 %. The 10-day window started at day three. No real lag phase / adaptation phase was noticeable.
With 3 %, no significant abiotic degradation of Naringin DHC was noticeable after 28 days of incubation.
The biodegradation of the item mixture in the toxicity control was found to be 66 % after 14 days of incubation. Thus, the demanded threshold value of 25 % is exceeded and the test item Naringin DHC can be identified as non toxic in a ready biodegradability test.
The reference item sodium benzoate was degraded to 81 % within the first 14 days.
Due to a biodegradation above 60 % within a 10-day-window, the test item Naringin DHC can be identified as readily biodegradable.
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