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EC number: 203-782-3 | CAS number: 110-60-1
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2009
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: test according to OECD301B under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Tetramethylenediamine
- EC Number:
- 203-782-3
- EC Name:
- Tetramethylenediamine
- Cas Number:
- 110-60-1
- Molecular formula:
- C4H12N2
- IUPAC Name:
- butane-1,4-diamine
- Details on test material:
- - Name of test material (as cited in study report): 1,4 diaminobutane in water
- Physical state: clear colorless liquid
- Analytical purity: 67,91% w/w
- Composition of test material, percentage of components: 67,91% w/w 1,4 diaminobutane in water
- Lot/batch No.: DAB 81
- Stability under test conditions: stable
- Storage condition of test material: at room temperature in dark under nitrogen
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- Fresh activated sludge from municipal sewage treatment plant: "Waterschap de Maaskant" at Den Bosch the Netherlands
- Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 32.5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium as described in OECD301B guideline
- Solubilising agent (type and concentration if used): Mili-RO water
- Test temperature: 21.3-24 °C
- pH: 7.2-8.1
- pH adjusted: yes
- Suspended solids concentration: 1% final volume of decanted inoculum (inoculum SS= 4.4 g/l, before use sludge allowed to settle for 80 min before decanting)
- Continuous darkness: no
- Other: IC < 5% of TC (mainly comning from test substance, 12 mg/l TOC)
TEST SYSTEM
- Number of culture flasks/concentration: 2 bottles test suspension, 2 bottles blank inoculum, 1 bottle positive control, 1 bottle toxicity control
- Method used to create aerobic conditions: sparging with synthetic air (CO2 < 1 ppm)
- Measuring equipment: titration with 0.05 M standarHCl and Phenolptalein as pH indicator
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: three CO2 absorbers with 100 ml 0.0125 M Ba(OH)2 in serie on the exit air line
SAMPLING
- Sampling frequency: every second or third day
- Sampling method: CO2-absorbers
- Sample storage before analysis: at room temperature in the dark under nitrogen
CONTROL AND BLANK SYSTEM
- Inoculum blank: only containing inoculum
- Toxicity control: test substance, reference substance and inoculum
- Positive control: reference substance and inoculum
STATISTICAL METHODS:
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- purity > 99,0%, Supplier Merck, Art no 1.06268.0250
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- >= 89
- Sampling time:
- 28 d
Any other information on results incl. tables
In the toxicity control more than 25% biodegradation occured within 14 days (71% based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. The positive control substance was biodegraded for 77% within 14 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- criteria of OECD301B and ISO standard 9439:
- Interpretation of results:
- readily biodegradable
- Conclusions:
- 1,4 diaminobutane (67%) is designated as readily biodegradable
- Executive summary:
The relative biodegradation values calculated from the measurements performed during the test period revealed 82% and 89% biodegradation of 1,4 diaminobutane (67%) for A and B, respectively. Furthermore, biodegradation of 1,4 diaminobutane (67%) of at least 60% was reached within a 10 -day window. In the toxicity control, 1,4 diaminobutane (67%) was found no to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study is considered to be valid. In conclusion 1,4 diaminobutane (67%) is designated a readily biodegradable
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