Tetramethylenediamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: test according to OECD301B under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tetramethylenediamine

EC Number:

203-782-3

EC Name:

Tetramethylenediamine

Cas Number:

110-60-1

Molecular formula:

C4H12N2

IUPAC Name:

butane-1,4-diamine

Details on test material:

- Name of test material (as cited in study report): 1,4 diaminobutane in water
- Physical state: clear colorless liquid
- Analytical purity: 67,91% w/w
- Composition of test material, percentage of components: 67,91% w/w 1,4 diaminobutane in water
- Lot/batch No.: DAB 81
- Stability under test conditions: stable
- Storage condition of test material: at room temperature in dark under nitrogen

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Fresh activated sludge from municipal sewage treatment plant: "Waterschap de Maaskant" at Den Bosch the Netherlands

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

32.5 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: mineral medium as described in OECD301B guideline
- Solubilising agent (type and concentration if used): Mili-RO water
- Test temperature: 21.3-24 °C
- pH: 7.2-8.1
- pH adjusted: yes
- Suspended solids concentration: 1% final volume of decanted inoculum (inoculum SS= 4.4 g/l, before use sludge allowed to settle for 80 min before decanting)
- Continuous darkness: no
- Other: IC < 5% of TC (mainly comning from test substance, 12 mg/l TOC)

TEST SYSTEM
- Number of culture flasks/concentration: 2 bottles test suspension, 2 bottles blank inoculum, 1 bottle positive control, 1 bottle toxicity control
- Method used to create aerobic conditions: sparging with synthetic air (CO2 < 1 ppm)
- Measuring equipment: titration with 0.05 M standarHCl and Phenolptalein as pH indicator
- Test performed in closed vessels due to significant volatility of test substance: yes
- Details of trap for CO2 and volatile organics if used: three CO2 absorbers with 100 ml 0.0125 M Ba(OH)2 in serie on the exit air line

SAMPLING
- Sampling frequency: every second or third day
- Sampling method: CO2-absorbers
- Sample storage before analysis: at room temperature in the dark under nitrogen

CONTROL AND BLANK SYSTEM
- Inoculum blank: only containing inoculum
- Toxicity control: test substance, reference substance and inoculum
- Positive control: reference substance and inoculum

STATISTICAL METHODS:

Reference substance

Reference substance:

acetic acid, sodium salt

Remarks:

purity > 99,0%, Supplier Merck, Art no 1.06268.0250

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

>= 89

Sampling time:

28 d

Any other information on results incl. tables

In the toxicity control more than 25% biodegradation occured within 14 days (71% based on ThCO2). Therefore, the test substance was assumed not to inhibit microbial activity. The positive control substance was biodegraded for 77% within 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

criteria of OECD301B and ISO standard 9439:

Interpretation of results:

readily biodegradable

Conclusions:

1,4 diaminobutane (67%) is designated as readily biodegradable

Executive summary:

The relative biodegradation values calculated from the measurements performed during the test period revealed 82% and 89% biodegradation of 1,4 diaminobutane (67%) for A and B, respectively. Furthermore, biodegradation of 1,4 diaminobutane (67%) of at least 60% was reached within a 10 -day window. In the toxicity control, 1,4 diaminobutane (67%) was found no to inhibit microbial activity. Since all criteria for acceptability of the test were met, this study is considered to be valid. In conclusion 1,4 diaminobutane (67%) is designated a readily biodegradable