Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-03-01 - 1990-03-20
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test follows guideline, some deficiences in reporting

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report Date:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
Akzo No.; 1-036-013
Product code: 56508
Sample code: 12
Batch No.: 900110
Certificate of analysis: 1990-03-05
Amine oxide content: 30.5%
Free amine: 0.3%
Free peroxide: 0.13%
pH (10+90 water): 7.1
Colour: <2 (Gard.)

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
n.a., measurement of extinction

Test solutions

Vehicle:
no
Details on test solutions:
no

Test organisms

Test organisms (species):
Pseudomonas putida
Details on inoculum:
Pseudomonas putida 50026

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
18 h
Post exposure observation period:
none

Test conditions

Hardness:
no data
Test temperature:
25 deg C
pH:
Potassium hydrogenphosphate KH2PO4/Di Potassium hydrogenphosphate K2HPO4 buffer
Dissolved oxygen:
no data
Salinity:
no
Nominal and measured concentrations:
Nominal test substance (mg/L): 100-500-1000-3000-10000, Control (mg/L): 0
Details on test conditions:
according guideline
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
18 h
Dose descriptor:
EC10
Effect conc.:
702 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition
Details on results:
graphical evaluation
Results with reference substance (positive control):
n.a.
Reported statistics and error estimates:
n.a.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance has shown a low toxicity to Pseudomonas putida in an 18h growth inhibition test according DIN 38412, part 8 resulting in an EC10 (18h) of 2300 mg/L (nominal, test substance) or 702 mg/L (nominal, active ingredient).