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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18 February - 24 March 1990
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study performed under GLP and according to guideline with some deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990
Report date:
1990

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
yes
Remarks:
No measurements of the concentrations. No data on Daphnia culture.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
EC Number:
263-180-1
EC Name:
Ethanol, 2,2'-iminobis-, N-coco alkyl derivs., N-oxides
Cas Number:
61791-47-7
Molecular formula:
not applicable
IUPAC Name:
N,N-Bis(2-hydroxyethyl)-C12-18(even numbered) alkyl-1-amine oxides
Details on test material:
The test material was identified by name and specifications:
- chemical name: Cocobis(2-hydroxyethyl)amine oxide
- cas nr : 61791-47-7
- batch no: 900110 (CoA attached to the report):
Amine oxide: 30.5%
Free amine: 0.3%
Free peroxide: 0.13%
water: balance
CRL code T90-2-1.5

It was supplied by the study sponsor on 8 Feb 1990. Stored in the dark at room temperature.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
Liquid.
Stable at test concentrations (data on biodegradability available).
Water soluble at test concentrations.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
The test solutions were prepared from stock solutions of the test substance in deionized water. The stock solution was clear and homogeneous
(some foam was produced but disappeared after a short time). The test concentrations were prepared by addition of the respective amounts of the
stock solution to the DutchStandardWater. For the preparation of the test concentration a stock solution containing 0.3 g/L was used.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna (waterflea) originated from the continuous culture maintained at the research facilities. The animals used in the test were less than
24 hours old at the beginning of the test and were obtained from parent animals having an age of about 3 weeks.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
None

Test conditions

Hardness:
13 dH
Test temperature:
19.3 - 19.7 C
pH:
8.0 - 8.1
Dissolved oxygen:
8.8 - 9.1 mg/L
Nominal and measured concentrations:
Nominal concentrations:
0.46 - 1.0 - 2.1 - 4.6 - 10 mg/L
Details on test conditions:
400 mL glass beakers containing 250 mL of test medium were partly covered with a glass plate. Each beaker contained 5 animals. In the control 30
animals were tested, while 20 animals were tested per test concentration.
The diluting water used for the preparation of the test concentrations was a synthetic water: Dutch Standard Water (DSW): ph = 8.2 and a hardness of 13 dH. The test was carried out in a temperature controlled room. The light regime was 16 hours of ambient light per day, supplied by fluorescent
tubes. pH and oxygen concentrations were measured daily.

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
1.1 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mobility
Remarks on result:
other: 95% CI 0.9-1.3 mg/L
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 95% CI 2.9 - 4.3 mg/L
Details on results:
The NOEC after 48 hours amounted to 0.46 mg/L, whereas 10 mg/L produced 100% immobilization within 48 hours. The EC50 (48h) was calculated
to be 3.5 mg/L with 95% confidence limits of 2.9 - 4.3 mg/L.
Reported statistics and error estimates:
The EC50 was determined with an LC50 programme of Griffioen (RIZA, NL) based on a model of Kooyman (1981).

Any other information on results incl. tables

Number of mobile animals

Conc substance

Test vessel

in mg/l

number

0 hours

24 hours

48 hours

1

5

5

5

2

5

5

5

3

5

5

5

control

4

5

5

5

5

5

5

5

6

5

5

5

total

30

30

30

1

5

5

5

2

5

5

5

0.46

3

5

5

5

4

5

5

5

total

20

20

20

1

5

5

5

2

5

5

4

1.0

3

5

5

5

4

5

5

5

total

20

20

19

1

5

5

5

2

5

5

5

2.1

3

5

5

4

4

5

5

5

total

20

20

19

1

5

5

1

2

5

5

0

4.6

3

5

4

1

4

5

5

3

total

20

19

5

1

5

4

0

2

5

3

0

10

3

5

5

0

4

5

4

0

total

20

16

0

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The EC50(48h) was calculated to be 3.5 mg/L with 95% confidence limits of 2.9 and 4.3 mg/L. The NOEC (48h) was 0.46 mg/L. If it is assumed that the product has a 100% pure active ingredient and that the toxicity is related to the active ingredient only, the EC50 and NOEC (48h) amount to 1.1 and 0.14 mg/L amine oxide respectively.
Executive summary:

A static study was performed according to OECD203 and GLP to investigate the acute toxicity of the test item to the aquatic invertebrate Daphnia magna. Based on nominal concentrations only, the EC50 was calculated to be 3.5 mg/L with 95% confidence limits of 2.9 and 4.3 mg/L. The NOEC after 48h was 0.46 mg/L.