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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: well documented GLP study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1985
Report date:
1985

Materials and methods

Principles of method if other than guideline:
according to Ames, B.N. et al.: Mutat. Res. 31, 347-364, 1975
GLP compliance:
yes (incl. QA statement)
Remarks:
testing lab.
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
4'-hydroxyacetophenone
EC Number:
202-802-8
EC Name:
4'-hydroxyacetophenone
Cas Number:
99-93-4
Molecular formula:
C8H8O2
IUPAC Name:
1-(4-hydroxyphenyl)ethan-1-one
Details on test material:
Name of the test substance used in the study report: C-800, SN-2592

Method

Target gene:
several S. typhimurium strains
Species / strain
Species / strain / cell type:
other: Salmonella typhimurium strains TA 98, 100, 1535, 1537, 1538
Metabolic activation:
with and without
Metabolic activation system:
rat liver activation system
Test concentrations with justification for top dose:
1.0, 10, 100, 500, 1000, 2500, 5000, 10000 ug/plate
Vehicle / solvent:
DMSO
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
yes
Positive controls:
yes
Positive control substance:
other: without metabolic activation: sodium azide, 2-Nitrofluorene, 9-aminoacridine; with metabolic activation: 2-aminoanthracene
Details on test system and experimental conditions:
Doses for the mutagenicity assay were selected from a preliminary range-finding study was performed with tested strain TA 100 at 14 dose levels (i.e from 1.22 to 10000 ug/plate). In this study the test material was toxic to the indicator strain at and above 2500 ug/plate as evidenced by the reduced number of revertants on the minimal media plates. In the mutagenicity assay eight dose leves from 1 to 10000 ug/plate were tested with and without metabolic activation, using one plate per dose level in the agar incorporation method. As vehicel DMSO was chosen. S-9 mix was prepared from livers of Aroclor induced male Sprague-Dawley rats.
Plates were incubated at 37° C for approx. 48 hours.

Results and discussion

Test results
Species / strain:
other: S. typhimurium strains TA 98, 100, 1535, 1537, 1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
other: >= 2500 ug/plate
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

The test substance did not exhibit genetic activity in any of the assays conducted in the present study and was not mutagenic under these test conditions.