2-Propenenitrile, reaction products with ethylenediamine, hydrogenated

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment. For read across justification please refer to IUCLID chapter 13.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Report date:

1977

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

only one dose level

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

N-(2-aminoethyl)-1,3-propanediamine

EC Number:

236-882-0

EC Name:

N-(2-aminoethyl)-1,3-propanediamine

Cas Number:

13531-52-7

Molecular formula:

C5H15N3

IUPAC Name:

N1-(2-Aminoethyl)-1,3-propanediamine

Details on test material:

- Name of test material (as cited in study report): N-Mono-(aminopropyl)1,2-diaminoaethan (N-3-Amin)
- Analytical purity: 98 %

Test animals

Species:

rabbit

Strain:

Vienna White

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: male: 3,29 kg (mean); female: 3,44 kg (mean)

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

TEST SITE
- Area of exposure: 50 cm²

REMOVAL OF TEST SUBSTANCE
- Washing (if done):

TEST MATERIAL
- Concentration (if solution): 100 %

Duration of exposure:

once

Doses:

200 µl/kg

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes

Statistics:

None

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

184 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Calculated from 0.2 ml/kg assuming test substance density of 0.92 g/ml

Mortality:

2 male animals and 1 female animal died after 7 days.

Clinical signs:

other: slight apathy in all animals, apathy in 3 animals, urine tinged with blood in 3 of the 3 animals that died

Gross pathology:

- Animals that died spontaneously: Kidney (renal pelvis and urinary bladder filled with blood); Liver (focal mass necroses); lung (severe edema); heart (dilatation bilaterally)
- Animals sacrificed after 14 days: no adverse effects observed

Other findings:

Necrosis was observed in all animals

Any other information on results incl. tables

Mortality

Dose (µl/kg)	Conc. (%)	No. of Animals	died within1 h	24 h	48 h	7 d	14 d
200	100	3 male	0/3	0/3	0/3	2/3	2/3
200	100	3 female	0/3	0/3	0/3	1/3	1/3