Dibenzo[d,f][1,3,2]dioxaphosphepin, 6,6'-[[3,3',5,5'-tetrakis(1,1-dimethylethyl)[1,1'-biphenyl]-2,2'-diyl]bis(oxy)]bis-

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Principles of method if other than guideline:

The animals were maintained on appropriate commercial diet and municipal water. Both are available ad libitum except during periods of fasting or manipulation. Dosage levels for the toxicity tests normally differ by a factor of 2 in a geometric series, but may differ by other constant factors if required. The maximum dosage for the peroral and percutaneous tests is 16 ml/kg. Dosages are reduced until significant signs of toxicity are not observed. LD50's and the estimated LD50 slopes are calculated by the moving average method (Thompson, 1947; Weil, 1983) and are based on
a 14-day observation period. Animal weights are recorded at 0 days (before dose), 7 days and 14 days (just prior to sacrifice). At death or sacrifice, each animal is subjected to gross pathologic evaluation.

Peroral Intubation:
Sprague-Dawley albino rats, weighing between 200 and 300 g, receive the test material by stomach intubation with a ball- end stainless steel needle. The sample is injected through the needle by means of a syringe and doses are varied by adjusting the volume of the test material or its dilution.
The rats are fasted overnight before dosing. Five males and five females are included in each level used for LD50 calculations.

GLP compliance:

not specified

Test type:

fixed dose procedure

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

482-400-9

EC Name:

-

Cas Number:

138776-88-2

Molecular formula:

C52H56O6P2

IUPAC Name:

9-[(3,3',5,5'-tetra-tert-butyl-2'-{8,10-dioxa-9-phosphatricyclo[9.4.0.0²,⁷]pentadeca-1(11),2(7),3,5,12,14-hexaen-9-yloxy}-[1,1'-biphenyl]-2-yl)oxy]-8,10-dioxa-9-phosphatricyclo[9.4.0.0²,⁷]pentadeca-1(11),2,4,6,12,14-hexaene

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Doses:

25% (w/v) suspension in corn oil. Because of large dose volumes required, doses were divided into 2 portions and administered approximately one hour apart.
Applied doses were 16 g/kg for females and 8 and 16 g/kg for males.

No. of animals per sex per dose:

5

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

> 16 000 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

> 16 000 mg/kg bw

Based on:

test mat.

Any other information on results incl. tables

No animals died in any dose group.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Remarks:

Critieria used; EU

Conclusions:

There were no deaths in male and female rats at 16000 mg/kg.